Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20413 | 3' | -56.9 | NC_004688.1 | + | 67055 | 0.66 | 0.838366 |
Target: 5'- aCCGUGGCCCACCA-----AGCa-GGAc -3' miRNA: 3'- -GGCACUGGGUGGUgcaucUCGgaCCU- -5' |
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20413 | 3' | -56.9 | NC_004688.1 | + | 27003 | 0.66 | 0.838366 |
Target: 5'- uCCGUGACCCcggGCCACGgc--GCCa--- -3' miRNA: 3'- -GGCACUGGG---UGGUGCaucuCGGaccu -5' |
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20413 | 3' | -56.9 | NC_004688.1 | + | 85594 | 0.66 | 0.821524 |
Target: 5'- cCCaUGACCCGCU-CGUccgccGCCUGGAg -3' miRNA: 3'- -GGcACUGGGUGGuGCAucu--CGGACCU- -5' |
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20413 | 3' | -56.9 | NC_004688.1 | + | 57307 | 0.67 | 0.785819 |
Target: 5'- uUCGUGAucUCCACgGCGUgcggaaAGAGCUgGGAg -3' miRNA: 3'- -GGCACU--GGGUGgUGCA------UCUCGGaCCU- -5' |
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20413 | 3' | -56.9 | NC_004688.1 | + | 6690 | 0.67 | 0.773708 |
Target: 5'- gCCGcGGCCCACCAggaucucuuCGgGGccccaggcggugauGGCCUGGAu -3' miRNA: 3'- -GGCaCUGGGUGGU---------GCaUC--------------UCGGACCU- -5' |
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20413 | 3' | -56.9 | NC_004688.1 | + | 42891 | 0.68 | 0.728265 |
Target: 5'- cCCGUGACUcgaCACCuCGUAGAggcccucguaGCCgGGGg -3' miRNA: 3'- -GGCACUGG---GUGGuGCAUCU----------CGGaCCU- -5' |
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20413 | 3' | -56.9 | NC_004688.1 | + | 51097 | 0.68 | 0.717326 |
Target: 5'- cCCGUGAaaccguuCCCGcCCGCGaGGGguggauucucccGCCUGGAu -3' miRNA: 3'- -GGCACU-------GGGU-GGUGCaUCU------------CGGACCU- -5' |
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20413 | 3' | -56.9 | NC_004688.1 | + | 95929 | 0.68 | 0.698229 |
Target: 5'- gUCGUGcgcgcgccagGCCCACCGCaUGGAGCCg--- -3' miRNA: 3'- -GGCAC----------UGGGUGGUGcAUCUCGGaccu -5' |
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20413 | 3' | -56.9 | NC_004688.1 | + | 17038 | 0.68 | 0.697218 |
Target: 5'- gCCGUuugGGCCCgACCagggcuuGCGUGGGGCaUGGAu -3' miRNA: 3'- -GGCA---CUGGG-UGG-------UGCAUCUCGgACCU- -5' |
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20413 | 3' | -56.9 | NC_004688.1 | + | 13254 | 0.72 | 0.495844 |
Target: 5'- aCCGggagGACCUugcuauCCACGUAGGGUCcGGGg -3' miRNA: 3'- -GGCa---CUGGGu-----GGUGCAUCUCGGaCCU- -5' |
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20413 | 3' | -56.9 | NC_004688.1 | + | 5836 | 1.11 | 0.001417 |
Target: 5'- cCCGUGACCCACCACGUAGAGCCUGGAg -3' miRNA: 3'- -GGCACUGGGUGGUGCAUCUCGGACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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