Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20414 | 3' | -59 | NC_004688.1 | + | 14438 | 0.66 | 0.727149 |
Target: 5'- uUCAAGCCUGGGCCcGAagguuaCGGUCGc-- -3' miRNA: 3'- -AGUUUGGGUCCGGcCUg-----GCCAGCcau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 6327 | 0.66 | 0.717234 |
Target: 5'- gCGAuaACCCAGGCCGG---GGUUGGc- -3' miRNA: 3'- aGUU--UGGGUCCGGCCuggCCAGCCau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 89905 | 0.67 | 0.666748 |
Target: 5'- cUCGAACgCGGGUgGGugCGG-CGGc- -3' miRNA: 3'- -AGUUUGgGUCCGgCCugGCCaGCCau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 35632 | 0.67 | 0.636047 |
Target: 5'- aCucACCCAGcCCGGGCCGGU-GGc- -3' miRNA: 3'- aGuuUGGGUCcGGCCUGGCCAgCCau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 33975 | 0.68 | 0.595104 |
Target: 5'- gUCAAAUCCGGGUCaGGgucaggcaGCgGGUCGGg- -3' miRNA: 3'- -AGUUUGGGUCCGG-CC--------UGgCCAGCCau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 76053 | 0.68 | 0.574766 |
Target: 5'- -gAAGCCCGcGGCCcucCCGGUCGGg- -3' miRNA: 3'- agUUUGGGU-CCGGccuGGCCAGCCau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 28364 | 0.68 | 0.564655 |
Target: 5'- ---cGCCagguGGCCGGACUGGgugaCGGUGg -3' miRNA: 3'- aguuUGGgu--CCGGCCUGGCCa---GCCAU- -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 28404 | 0.69 | 0.524744 |
Target: 5'- cCAggUCCGGgaaGCCGGGCUGGUCGc-- -3' miRNA: 3'- aGUuuGGGUC---CGGCCUGGCCAGCcau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 43480 | 0.69 | 0.514928 |
Target: 5'- uUCAGguuGCCCAGGaCGGugCGGgcgCGGc- -3' miRNA: 3'- -AGUU---UGGGUCCgGCCugGCCa--GCCau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 5979 | 0.69 | 0.514928 |
Target: 5'- cUCGcGCCCGGGuuGGGCCcGUCGa-- -3' miRNA: 3'- -AGUuUGGGUCCggCCUGGcCAGCcau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 73594 | 0.69 | 0.505188 |
Target: 5'- aUCGAugCgGGGCCGGGCCcG-CGGg- -3' miRNA: 3'- -AGUUugGgUCCGGCCUGGcCaGCCau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 96875 | 0.71 | 0.421615 |
Target: 5'- -aAAACCCAauuCCGGaguuGCCGGUCGGUGg -3' miRNA: 3'- agUUUGGGUcc-GGCC----UGGCCAGCCAU- -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 27654 | 0.71 | 0.421615 |
Target: 5'- aUCGAAgCCGaGCCGGAUagCGGUCGGg- -3' miRNA: 3'- -AGUUUgGGUcCGGCCUG--GCCAGCCau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 91703 | 0.71 | 0.395652 |
Target: 5'- cCGGACCCAGGCCGaACCGuuGUCGa-- -3' miRNA: 3'- aGUUUGGGUCCGGCcUGGC--CAGCcau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 6774 | 0.73 | 0.33167 |
Target: 5'- gCAGGCCUGGGCCucuucGGACUGGuUCGGg- -3' miRNA: 3'- aGUUUGGGUCCGG-----CCUGGCC-AGCCau -5' |
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20414 | 3' | -59 | NC_004688.1 | + | 6024 | 1.06 | 0.001573 |
Target: 5'- gUCAAACCCAGGCCGGACCGGUCGGUAc -3' miRNA: 3'- -AGUUUGGGUCCGGCCUGGCCAGCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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