Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20414 | 5' | -63.8 | NC_004688.1 | + | 30388 | 0.66 | 0.490982 |
Target: 5'- gGUCGCCaggGCGCCCGGGaacaggucGcGGCCg -3' miRNA: 3'- -UAGUGGgagCGCGGGCCCaa------C-CCGGg -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 21515 | 0.66 | 0.490982 |
Target: 5'- uUCGCCCUUGauggugcgGCCCGGGaguucGGuGCCg -3' miRNA: 3'- uAGUGGGAGCg-------CGGGCCCaa---CC-CGGg -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 17059 | 0.66 | 0.48173 |
Target: 5'- aGUCGCCUUgGCGaCCuCGccgccguUUGGGCCCg -3' miRNA: 3'- -UAGUGGGAgCGC-GG-GCcc-----AACCCGGG- -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 58822 | 0.66 | 0.48173 |
Target: 5'- cAUCugCCUCGuCG-CCGGcGccgGGGCCa -3' miRNA: 3'- -UAGugGGAGC-GCgGGCC-Caa-CCCGGg -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 42703 | 0.66 | 0.48173 |
Target: 5'- cGUCGCCUUCGauguaGUCaaGGacgcaUUGGGCCCa -3' miRNA: 3'- -UAGUGGGAGCg----CGGgcCC-----AACCCGGG- -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 62898 | 0.66 | 0.463485 |
Target: 5'- cUCGCCCUUGUGaggCGGG-UGGGCg- -3' miRNA: 3'- uAGUGGGAGCGCgg-GCCCaACCCGgg -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 26351 | 0.66 | 0.454498 |
Target: 5'- -gCGCCCaUgGCGCCCuGGaUGGcagcGCCCg -3' miRNA: 3'- uaGUGGG-AgCGCGGGcCCaACC----CGGG- -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 33624 | 0.66 | 0.445608 |
Target: 5'- -cCGCCa--GCGCCCGGGUcgaGGGUg- -3' miRNA: 3'- uaGUGGgagCGCGGGCCCAa--CCCGgg -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 28526 | 0.67 | 0.436815 |
Target: 5'- --aGCCCUC-UGaCCCGGG-UGGGUCUu -3' miRNA: 3'- uagUGGGAGcGC-GGGCCCaACCCGGG- -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 38987 | 0.67 | 0.428123 |
Target: 5'- cUUACCCccgggCGCGCCgGGGauaacgacGGCCCc -3' miRNA: 3'- uAGUGGGa----GCGCGGgCCCaac-----CCGGG- -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 68912 | 0.67 | 0.419534 |
Target: 5'- -gCACCUUCaGaCGCCaaGGcgUGGGCCCu -3' miRNA: 3'- uaGUGGGAG-C-GCGGgcCCa-ACCCGGG- -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 57219 | 0.68 | 0.370274 |
Target: 5'- -cCACCgUCGuCGCaucacCCGGGcgGGGCCa -3' miRNA: 3'- uaGUGGgAGC-GCG-----GGCCCaaCCCGGg -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 46767 | 0.68 | 0.354755 |
Target: 5'- gAUCACUCgCGggauCGCCCGGc--GGGCCCu -3' miRNA: 3'- -UAGUGGGaGC----GCGGGCCcaaCCCGGG- -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 37311 | 0.69 | 0.325115 |
Target: 5'- uUCAaCCUCGuCG-CCGGuGUUGGuGCCCg -3' miRNA: 3'- uAGUgGGAGC-GCgGGCC-CAACC-CGGG- -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 24221 | 0.69 | 0.29535 |
Target: 5'- -gCGuCCgUCGCGCCCGuggggaccguugccGGUgcccgGGGCCCc -3' miRNA: 3'- uaGU-GGgAGCGCGGGC--------------CCAa----CCCGGG- -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 79854 | 0.72 | 0.204648 |
Target: 5'- -aCACCCacggGCGCCCGucGGccgagUGGGCCCa -3' miRNA: 3'- uaGUGGGag--CGCGGGC--CCa----ACCCGGG- -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 42868 | 0.72 | 0.195003 |
Target: 5'- --gGCCCUCGUaGCCgGGGgcUGGGCgCCa -3' miRNA: 3'- uagUGGGAGCG-CGGgCCCa-ACCCG-GG- -5' |
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20414 | 5' | -63.8 | NC_004688.1 | + | 5986 | 1.09 | 0.000377 |
Target: 5'- cAUCACCCUCGCGCCCGGGUUGGGCCCg -3' miRNA: 3'- -UAGUGGGAGCGCGGGCCCAACCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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