Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20415 | 3' | -54.6 | NC_004688.1 | + | 36093 | 0.66 | 0.888396 |
Target: 5'- cACggUUguGCGCC--ACgGCGGCCAu -3' miRNA: 3'- cUGuaAAguCGCGGcuUGaCGCCGGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 9616 | 0.66 | 0.881178 |
Target: 5'- cACGUUUC-GCGCCGAuCUcGUcgaaGGCCAc -3' miRNA: 3'- cUGUAAAGuCGCGGCUuGA-CG----CCGGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 24009 | 0.66 | 0.881178 |
Target: 5'- ----cUUCAGCcagGCCGGgauACUGaCGGCCGc -3' miRNA: 3'- cuguaAAGUCG---CGGCU---UGAC-GCCGGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 107540 | 0.66 | 0.881178 |
Target: 5'- aACGUUgUCcGCuGCgGAACgUGCGGCCGa -3' miRNA: 3'- cUGUAA-AGuCG-CGgCUUG-ACGCCGGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 88866 | 0.66 | 0.87372 |
Target: 5'- cGACAaugUCGGCuaCGAuCUGCaGCCAc -3' miRNA: 3'- -CUGUaa-AGUCGcgGCUuGACGcCGGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 97282 | 0.66 | 0.87372 |
Target: 5'- aGCggUUUGGCGUCGu-CUGCGGCgAg -3' miRNA: 3'- cUGuaAAGUCGCGGCuuGACGCCGgU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 24292 | 0.66 | 0.865245 |
Target: 5'- aGGCccgcgUCAGCaggugccggGCCGAugcccgugguagcGCUGCGGCCc -3' miRNA: 3'- -CUGuaa--AGUCG---------CGGCU-------------UGACGCCGGu -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 83481 | 0.66 | 0.863675 |
Target: 5'- aGACAUcguuucggcccugcUUCAGCGCC-AACgagGCcGCCAa -3' miRNA: 3'- -CUGUA--------------AAGUCGCGGcUUGa--CGcCGGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 15218 | 0.67 | 0.858106 |
Target: 5'- cGugGUcaaCGGCGCCGcGCcGCGGCaCAu -3' miRNA: 3'- -CugUAaa-GUCGCGGCuUGaCGCCG-GU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 78811 | 0.67 | 0.849962 |
Target: 5'- uGACuugccUAGC-CCGGGCgggGCGGCCAg -3' miRNA: 3'- -CUGuaaa-GUCGcGGCUUGa--CGCCGGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 56869 | 0.67 | 0.841604 |
Target: 5'- gGACuaagccCGGCGUCGAuCaGCGGCCGa -3' miRNA: 3'- -CUGuaaa--GUCGCGGCUuGaCGCCGGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 65688 | 0.67 | 0.841604 |
Target: 5'- ------cCGGCGCCuAGCuUGCGGCCu -3' miRNA: 3'- cuguaaaGUCGCGGcUUG-ACGCCGGu -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 74695 | 0.67 | 0.841604 |
Target: 5'- aGGCGggugccgUCGGCGCCGAuGCcgauauCGGCCAg -3' miRNA: 3'- -CUGUaa-----AGUCGCGGCU-UGac----GCCGGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 49153 | 0.68 | 0.80619 |
Target: 5'- aGugAUgagguGCGCCGcGCcGCGGCCGa -3' miRNA: 3'- -CugUAaagu-CGCGGCuUGaCGCCGGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 51681 | 0.68 | 0.768041 |
Target: 5'- cGCggUUCAGCGCCGAcACcgGCGGaCGu -3' miRNA: 3'- cUGuaAAGUCGCGGCU-UGa-CGCCgGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 51762 | 0.69 | 0.738014 |
Target: 5'- cGACGUUggCGGUGCUGGaugcggagaagGCUGCGGUUg -3' miRNA: 3'- -CUGUAAa-GUCGCGGCU-----------UGACGCCGGu -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 23176 | 0.69 | 0.727791 |
Target: 5'- ------cCAGCGCCGccuGCUggGCGGCCGg -3' miRNA: 3'- cuguaaaGUCGCGGCu--UGA--CGCCGGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 11597 | 0.72 | 0.579814 |
Target: 5'- gGGCG----AGCGCCucaAACUGCGGCCAg -3' miRNA: 3'- -CUGUaaagUCGCGGc--UUGACGCCGGU- -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 26181 | 0.77 | 0.304632 |
Target: 5'- gGGCAUgcCGGUGCCGGGCUGCuGCCu -3' miRNA: 3'- -CUGUAaaGUCGCGGCUUGACGcCGGu -5' |
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20415 | 3' | -54.6 | NC_004688.1 | + | 6441 | 1.09 | 0.002337 |
Target: 5'- cGACAUUUCAGCGCCGAACUGCGGCCAg -3' miRNA: 3'- -CUGUAAAGUCGCGGCUUGACGCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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