Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20416 | 3' | -58.4 | NC_004688.1 | + | 54399 | 0.66 | 0.743298 |
Target: 5'- --cCCGGCCGguCUCGGCGa------ -3' miRNA: 3'- guuGGCCGGCguGAGCCGCccaaucu -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 52804 | 0.66 | 0.733511 |
Target: 5'- -uGCUGGCCGCGCUCGccccaucggauGCGG---AGAu -3' miRNA: 3'- guUGGCCGGCGUGAGC-----------CGCCcaaUCU- -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 29355 | 0.66 | 0.723634 |
Target: 5'- uGGCCgGGCCGaucgccacccCGCgCGGCGGGaaUUGGAc -3' miRNA: 3'- gUUGG-CCGGC----------GUGaGCCGCCC--AAUCU- -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 103236 | 0.67 | 0.693567 |
Target: 5'- uCAGCgaaUGGCCGCugUCGGuCGaGGUcaAGAu -3' miRNA: 3'- -GUUG---GCCGGCGugAGCC-GC-CCAa-UCU- -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 93262 | 0.67 | 0.683429 |
Target: 5'- gCAGCCGGCCGUcuuCUUugGuGCGGGUg--- -3' miRNA: 3'- -GUUGGCCGGCGu--GAG--C-CGCCCAaucu -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 65906 | 0.67 | 0.683429 |
Target: 5'- aAACCGGCaCcCACUcCGGUGGGUc--- -3' miRNA: 3'- gUUGGCCG-GcGUGA-GCCGCCCAaucu -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 34350 | 0.67 | 0.663034 |
Target: 5'- cCGGCUGGCCGguCUCcGcGCGGGa---- -3' miRNA: 3'- -GUUGGCCGGCguGAG-C-CGCCCaaucu -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 55806 | 0.67 | 0.652795 |
Target: 5'- gCGACCGGCgGCuagCGGCgcucgacacccgGGGUUGGc -3' miRNA: 3'- -GUUGGCCGgCGugaGCCG------------CCCAAUCu -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 74802 | 0.68 | 0.642539 |
Target: 5'- --cCCaGCCGauCugUCGGCGGGUgGGGg -3' miRNA: 3'- guuGGcCGGC--GugAGCCGCCCAaUCU- -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 78696 | 0.69 | 0.560848 |
Target: 5'- ---gUGGCCGCAUcgaUCGGCGGGa---- -3' miRNA: 3'- guugGCCGGCGUG---AGCCGCCCaaucu -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 67593 | 0.7 | 0.491808 |
Target: 5'- --gUCGGCgGCGgUCGGCGGGcugAGGu -3' miRNA: 3'- guuGGCCGgCGUgAGCCGCCCaa-UCU- -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 31662 | 0.71 | 0.435912 |
Target: 5'- gAACUGGCCGCGCagGGCGGa----- -3' miRNA: 3'- gUUGGCCGGCGUGagCCGCCcaaucu -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 63922 | 0.73 | 0.367449 |
Target: 5'- uGGCCGGggaCCGC-CUCGGCGGGg---- -3' miRNA: 3'- gUUGGCC---GGCGuGAGCCGCCCaaucu -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 5129 | 0.75 | 0.28605 |
Target: 5'- --cCCGGcCCGCAUUgCGGCGGGUgguGAa -3' miRNA: 3'- guuGGCC-GGCGUGA-GCCGCCCAau-CU- -5' |
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20416 | 3' | -58.4 | NC_004688.1 | + | 8517 | 1.08 | 0.001419 |
Target: 5'- uCAACCGGCCGCACUCGGCGGGUUAGAg -3' miRNA: 3'- -GUUGGCCGGCGUGAGCCGCCCAAUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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