Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20417 | 3' | -56.7 | NC_004688.1 | + | 74612 | 0.66 | 0.849132 |
Target: 5'- gCCG-AUGGACUUCCGCcagggcuGUUCUUUg -3' miRNA: 3'- aGGCuUGCCUGGAGGCGcu-----CAAGGAG- -5' |
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20417 | 3' | -56.7 | NC_004688.1 | + | 22506 | 0.66 | 0.849132 |
Target: 5'- gUCGggUGGAUCUCUuuauCGAGggCCUUg -3' miRNA: 3'- aGGCuuGCCUGGAGGc---GCUCaaGGAG- -5' |
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20417 | 3' | -56.7 | NC_004688.1 | + | 51607 | 0.66 | 0.841032 |
Target: 5'- -gUGAAUGuGAUUUCCGCGAcgGUUCgCUCg -3' miRNA: 3'- agGCUUGC-CUGGAGGCGCU--CAAG-GAG- -5' |
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20417 | 3' | -56.7 | NC_004688.1 | + | 12103 | 0.66 | 0.832739 |
Target: 5'- gCCG-GCGGAUgacgguggCUUCGCGGGUUugagCCUCg -3' miRNA: 3'- aGGCuUGCCUG--------GAGGCGCUCAA----GGAG- -5' |
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20417 | 3' | -56.7 | NC_004688.1 | + | 54399 | 0.66 | 0.832739 |
Target: 5'- cCCGGcCGG-UCUCgGCGAucUCCUCg -3' miRNA: 3'- aGGCUuGCCuGGAGgCGCUcaAGGAG- -5' |
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20417 | 3' | -56.7 | NC_004688.1 | + | 54204 | 0.66 | 0.824261 |
Target: 5'- cCCGGugGGucuugguguCCUUCuCGAcGUUCCUCa -3' miRNA: 3'- aGGCUugCCu--------GGAGGcGCU-CAAGGAG- -5' |
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20417 | 3' | -56.7 | NC_004688.1 | + | 13523 | 0.66 | 0.824261 |
Target: 5'- cUCCGGAUaGGCCUgCGCGAcGUcguugacggCCUCg -3' miRNA: 3'- -AGGCUUGcCUGGAgGCGCU-CAa--------GGAG- -5' |
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20417 | 3' | -56.7 | NC_004688.1 | + | 95550 | 0.67 | 0.797794 |
Target: 5'- --gGAGCGG-CCUCCGaagcuugggcUGGGUUCCUa -3' miRNA: 3'- aggCUUGCCuGGAGGC----------GCUCAAGGAg -5' |
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20417 | 3' | -56.7 | NC_004688.1 | + | 87866 | 0.67 | 0.796887 |
Target: 5'- gCCGaAGCGccuugauGACCccaguguucUCUGCGAGUUCUUCg -3' miRNA: 3'- aGGC-UUGC-------CUGG---------AGGCGCUCAAGGAG- -5' |
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20417 | 3' | -56.7 | NC_004688.1 | + | 63722 | 0.69 | 0.680738 |
Target: 5'- gUgGAGCGGGCCUCCGCcAGguaUUCg -3' miRNA: 3'- aGgCUUGCCUGGAGGCGcUCaagGAG- -5' |
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20417 | 3' | -56.7 | NC_004688.1 | + | 9120 | 1.1 | 0.001754 |
Target: 5'- gUCCGAACGGACCUCCGCGAGUUCCUCu -3' miRNA: 3'- -AGGCUUGCCUGGAGGCGCUCAAGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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