Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20417 | 5' | -55.5 | NC_004688.1 | + | 64034 | 0.66 | 0.879712 |
Target: 5'- gGCAGGGAccugacacgGCCGagcGAGCUggccucauaccCCGGAAGg -3' miRNA: 3'- -CGUUCCU---------UGGCgu-CUCGGa----------GGCCUUC- -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 23149 | 0.66 | 0.872331 |
Target: 5'- -aGAGGGAUUGgAGGGCCUCgGcGAAc -3' miRNA: 3'- cgUUCCUUGGCgUCUCGGAGgC-CUUc -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 75594 | 0.66 | 0.872331 |
Target: 5'- uGCGAcGGGgugucggccGCCGCGGAcuCCUCCGGcuGa -3' miRNA: 3'- -CGUU-CCU---------UGGCGUCUc-GGAGGCCuuC- -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 14908 | 0.66 | 0.864725 |
Target: 5'- aCGAGG--CCGUGGAGCUUCCGcAGGc -3' miRNA: 3'- cGUUCCuuGGCGUCUCGGAGGCcUUC- -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 4794 | 0.66 | 0.856898 |
Target: 5'- -aAAGGAucagGCCGgccaacucCAGGGCCUCCaGGAu- -3' miRNA: 3'- cgUUCCU----UGGC--------GUCUCGGAGG-CCUuc -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 10616 | 0.66 | 0.848857 |
Target: 5'- uCGAGGuGCCGguGAGgaUCUUCGGGAu -3' miRNA: 3'- cGUUCCuUGGCguCUC--GGAGGCCUUc -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 89800 | 0.67 | 0.832164 |
Target: 5'- aGCGAugucGGGGCCGC---GCCagCCGGGAGg -3' miRNA: 3'- -CGUU----CCUUGGCGucuCGGa-GGCCUUC- -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 61828 | 0.67 | 0.832164 |
Target: 5'- uGCGGcGGuaguuGCCGCGGGGCUcgUCGGAGc -3' miRNA: 3'- -CGUU-CCu----UGGCGUCUCGGa-GGCCUUc -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 84375 | 0.67 | 0.823527 |
Target: 5'- cGCGAaugcuGGucCCGUGGAGCUUCUGGGu- -3' miRNA: 3'- -CGUU-----CCuuGGCGUCUCGGAGGCCUuc -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 72823 | 0.67 | 0.805712 |
Target: 5'- uGCAuuGGGACCaGCAGAucuucuugGCCUCCGcGGc- -3' miRNA: 3'- -CGUu-CCUUGG-CGUCU--------CGGAGGC-CUuc -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 25066 | 0.68 | 0.777774 |
Target: 5'- cGCcGGGGACUGCccuGGAGCCgCCaucgcgacuggGGAAGg -3' miRNA: 3'- -CGuUCCUUGGCG---UCUCGGaGG-----------CCUUC- -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 101070 | 0.69 | 0.738652 |
Target: 5'- aGCu-GGAGcCCGUAGAGgUaCCGGAGGa -3' miRNA: 3'- -CGuuCCUU-GGCGUCUCgGaGGCCUUC- -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 32523 | 0.7 | 0.645879 |
Target: 5'- aCAAGGu-UUGCAGAaUCUCCGGAAGg -3' miRNA: 3'- cGUUCCuuGGCGUCUcGGAGGCCUUC- -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 54283 | 0.7 | 0.635391 |
Target: 5'- cCGGGGAACaC-CAGGGCgUCCGGAu- -3' miRNA: 3'- cGUUCCUUG-GcGUCUCGgAGGCCUuc -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 99291 | 0.7 | 0.635391 |
Target: 5'- uGCcaguGGGGGCgCGCAGcGCCaUCCGGAu- -3' miRNA: 3'- -CGu---UCCUUG-GCGUCuCGG-AGGCCUuc -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 25736 | 0.7 | 0.624899 |
Target: 5'- ---cGGGGCCGCcuugGGGGCCgCCGGAGc -3' miRNA: 3'- cguuCCUUGGCG----UCUCGGaGGCCUUc -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 41800 | 0.7 | 0.624899 |
Target: 5'- cCAGGuGACCGCguuGGGGuCCUCCGGGAu -3' miRNA: 3'- cGUUCcUUGGCG---UCUC-GGAGGCCUUc -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 87474 | 0.72 | 0.562351 |
Target: 5'- -aGGGuGGAgCGCAGAuaCUCCGGAAGg -3' miRNA: 3'- cgUUC-CUUgGCGUCUcgGAGGCCUUC- -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 24325 | 0.72 | 0.531668 |
Target: 5'- gGCAAcGGAACgucaUGCGGAGCC-CaCGGAGGa -3' miRNA: 3'- -CGUU-CCUUG----GCGUCUCGGaG-GCCUUC- -5' |
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20417 | 5' | -55.5 | NC_004688.1 | + | 15594 | 0.74 | 0.443923 |
Target: 5'- uCGAGGAcauccgcaucGCCGCAGAaaaccGCCUCCGGu-- -3' miRNA: 3'- cGUUCCU----------UGGCGUCU-----CGGAGGCCuuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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