Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20418 | 5' | -57.9 | NC_004688.1 | + | 87738 | 0.65 | 0.785887 |
Target: 5'- uUgGUGCCCCUCauuguuccgaagugGggGCGcgucugGCCGAc -3' miRNA: 3'- cAgCGCGGGGAG--------------CuuCGCuua---CGGCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 63937 | 0.66 | 0.779375 |
Target: 5'- aUCGCGCCCCcacacuggcCGGggaccgccucGGCGGggcGUGCCGu -3' miRNA: 3'- cAGCGCGGGGa--------GCU----------UCGCU---UACGGCu -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 90960 | 0.66 | 0.779375 |
Target: 5'- gGUCGCGCUCCacUGcGGCGuacaggGCCGGg -3' miRNA: 3'- -CAGCGCGGGGa-GCuUCGCuua---CGGCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 81975 | 0.66 | 0.779375 |
Target: 5'- uUCG-GCUCCUCG-GGCGuggcGCCGAg -3' miRNA: 3'- cAGCgCGGGGAGCuUCGCuua-CGGCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 83287 | 0.66 | 0.750765 |
Target: 5'- -cCG-GCaCCCUCGAAuGCGGugcggauUGCCGAc -3' miRNA: 3'- caGCgCG-GGGAGCUU-CGCUu------ACGGCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 98507 | 0.66 | 0.741003 |
Target: 5'- --gGCGUCCgUCGAGGCGGGaaaGCCu- -3' miRNA: 3'- cagCGCGGGgAGCUUCGCUUa--CGGcu -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 28498 | 0.67 | 0.731146 |
Target: 5'- --aGCGCCaCCUgGccaucGGCGAGgggGCCGAa -3' miRNA: 3'- cagCGCGG-GGAgCu----UCGCUUa--CGGCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 22938 | 0.67 | 0.721201 |
Target: 5'- -gCGCGCCCCgaggggaCGAacggcagaucGGUGAGcauguUGCCGAu -3' miRNA: 3'- caGCGCGGGGa------GCU----------UCGCUU-----ACGGCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 27074 | 0.67 | 0.701087 |
Target: 5'- cUCGCGCCCUuguuaUCGAGGUGAuugaauagaggGUGCa-- -3' miRNA: 3'- cAGCGCGGGG-----AGCUUCGCU-----------UACGgcu -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 24216 | 0.67 | 0.701087 |
Target: 5'- cGUCGCGCCCg-UGggGaccguUGCCGGu -3' miRNA: 3'- -CAGCGCGGGgaGCuuCgcuu-ACGGCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 13923 | 0.67 | 0.680738 |
Target: 5'- uGUCGCGCUCCguaUCGuuGGCGc-UGCCGc -3' miRNA: 3'- -CAGCGCGGGG---AGCu-UCGCuuACGGCu -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 107215 | 0.68 | 0.649931 |
Target: 5'- aUCuCGUCCCUCGAugAGCGAGaGuuGAu -3' miRNA: 3'- cAGcGCGGGGAGCU--UCGCUUaCggCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 28704 | 0.68 | 0.649931 |
Target: 5'- --aGCGCCCCUUGAggagGGCGuacauguUGCCu- -3' miRNA: 3'- cagCGCGGGGAGCU----UCGCuu-----ACGGcu -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 8840 | 0.68 | 0.618994 |
Target: 5'- gGUCGCGUUUgUCGAGGCGg--GCCa- -3' miRNA: 3'- -CAGCGCGGGgAGCUUCGCuuaCGGcu -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 20833 | 0.7 | 0.537467 |
Target: 5'- aGUC-CGCaCCCUCGcggugccacaGAGCGGGcuUGCCGAc -3' miRNA: 3'- -CAGcGCG-GGGAGC----------UUCGCUU--ACGGCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 61667 | 0.7 | 0.50778 |
Target: 5'- -gCGCGCCaCCgauaaCGAAgGCGAggGCCGGa -3' miRNA: 3'- caGCGCGG-GGa----GCUU-CGCUuaCGGCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 75182 | 0.72 | 0.432431 |
Target: 5'- uGUCGCGgaUCUCGuugcuGCGGAUGCCGGu -3' miRNA: 3'- -CAGCGCggGGAGCuu---CGCUUACGGCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 56698 | 0.73 | 0.388779 |
Target: 5'- aUCGCGCauaCCgauucUGAAGaCGGAUGCCGAg -3' miRNA: 3'- cAGCGCGg--GGa----GCUUC-GCUUACGGCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 17319 | 0.84 | 0.068643 |
Target: 5'- -gUGCGCgCCCUCGAuGCGGAUGCCGGg -3' miRNA: 3'- caGCGCG-GGGAGCUuCGCUUACGGCU- -5' |
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20418 | 5' | -57.9 | NC_004688.1 | + | 9301 | 1.08 | 0.001613 |
Target: 5'- gGUCGCGCCCCUCGAAGCGAAUGCCGAc -3' miRNA: 3'- -CAGCGCGGGGAGCUUCGCUUACGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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