Results 21 - 34 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20419 | 3' | -51.4 | NC_004688.1 | + | 61982 | 0.69 | 0.932897 |
Target: 5'- cGGCGUcccGcacgccgccaaucgaAGAUCCCGAcguuGCCgCGCGGg -3' miRNA: 3'- -CCGUA---C---------------UCUAGGGCUuuu-UGG-GCGCC- -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 62965 | 0.74 | 0.726634 |
Target: 5'- cGGCGgcgGAGAUCaCCGcgu-GCuCCGCGGu -3' miRNA: 3'- -CCGUa--CUCUAG-GGCuuuuUG-GGCGCC- -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 68194 | 0.7 | 0.907362 |
Target: 5'- cGGCG-GAGAugUCCUGAcaggGGAAUCCGCcGGu -3' miRNA: 3'- -CCGUaCUCU--AGGGCU----UUUUGGGCG-CC- -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 73602 | 0.71 | 0.831718 |
Target: 5'- aGgGUGGGAUCgaugCGggGccgGGCCCGCGGg -3' miRNA: 3'- cCgUACUCUAGg---GCuuU---UUGGGCGCC- -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 74374 | 0.68 | 0.949226 |
Target: 5'- cGGCuccacacccggGAGAUgCCGAuGAugCCGuCGGa -3' miRNA: 3'- -CCGua---------CUCUAgGGCUuUUugGGC-GCC- -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 76983 | 0.68 | 0.958245 |
Target: 5'- aGGCGUGuGGGUCCuCGG--GugCCGCa- -3' miRNA: 3'- -CCGUAC-UCUAGG-GCUuuUugGGCGcc -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 84394 | 0.66 | 0.979278 |
Target: 5'- aGGCAgGGGA-CCCGGccgacgcGAAugcugguCCCGUGGa -3' miRNA: 3'- -CCGUaCUCUaGGGCU-------UUUu------GGGCGCC- -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 85470 | 0.7 | 0.892745 |
Target: 5'- uGGCGUGAaagCCCGAAuuacaguauccACCCGgGGa -3' miRNA: 3'- -CCGUACUcuaGGGCUUuu---------UGGGCgCC- -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 90814 | 0.72 | 0.786137 |
Target: 5'- gGGCGcgcUGcugccaCCGGGAGACCCGCGGa -3' miRNA: 3'- -CCGU---ACucuag-GGCUUUUUGGGCGCC- -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 96298 | 0.7 | 0.894124 |
Target: 5'- aGGCGaaAGGUCCCcggcc-CCCGCGGg -3' miRNA: 3'- -CCGUacUCUAGGGcuuuuuGGGCGCC- -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 98865 | 0.71 | 0.831718 |
Target: 5'- cGCGUGAaguggcuucGAUCCUGcGAAgcggccacgGCCCGCGGc -3' miRNA: 3'- cCGUACU---------CUAGGGCuUUU---------UGGGCGCC- -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 101279 | 0.73 | 0.736831 |
Target: 5'- gGGCAagaaUGAGGUCCUGAcguugcAGACgCUGCGGu -3' miRNA: 3'- -CCGU----ACUCUAGGGCUu-----UUUG-GGCGCC- -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 101961 | 0.66 | 0.985589 |
Target: 5'- uGGCAUGAGAgCCgaCGAGGuAACCauCGGu -3' miRNA: 3'- -CCGUACUCUaGG--GCUUU-UUGGgcGCC- -5' |
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20419 | 3' | -51.4 | NC_004688.1 | + | 110687 | 0.7 | 0.88642 |
Target: 5'- uGGCAUGGcGAUUgCGAccgccugAGAGCCaCGUGGa -3' miRNA: 3'- -CCGUACU-CUAGgGCU-------UUUUGG-GCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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