Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20419 | 5' | -65 | NC_004688.1 | + | 70142 | 0.66 | 0.412701 |
Target: 5'- aGUCCGUGGGgaaugucaCCCGCCCgcggGGAUaGUCa-- -3' miRNA: 3'- -CAGGCGCCC--------GGGCGGG----CCUA-CAGcga -5' |
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20419 | 5' | -65 | NC_004688.1 | + | 5347 | 0.66 | 0.404392 |
Target: 5'- gGUCCGCGGGUCCa--CGc-UGUCGCUg -3' miRNA: 3'- -CAGGCGCCCGGGcggGCcuACAGCGA- -5' |
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20419 | 5' | -65 | NC_004688.1 | + | 73833 | 0.67 | 0.396191 |
Target: 5'- -aCCGgGGGCUaauuCCCGGAUGUUGg- -3' miRNA: 3'- caGGCgCCCGGgc--GGGCCUACAGCga -5' |
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20419 | 5' | -65 | NC_004688.1 | + | 94817 | 0.67 | 0.388096 |
Target: 5'- -cCCGCGuaucGCUCGCCCGGAg--CGCc -3' miRNA: 3'- caGGCGCc---CGGGCGGGCCUacaGCGa -5' |
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20419 | 5' | -65 | NC_004688.1 | + | 43720 | 0.67 | 0.364475 |
Target: 5'- -cCCGCGGGCCaggggcgcaGCUgGGAugccuUGUUGCg -3' miRNA: 3'- caGGCGCCCGGg--------CGGgCCU-----ACAGCGa -5' |
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20419 | 5' | -65 | NC_004688.1 | + | 30921 | 0.67 | 0.356826 |
Target: 5'- ---gGcCGGGCCCGCCCGGA--UCGa- -3' miRNA: 3'- caggC-GCCCGGGCGGGCCUacAGCga -5' |
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20419 | 5' | -65 | NC_004688.1 | + | 12085 | 0.68 | 0.341867 |
Target: 5'- cUUCGCGGGUUugaGCCUcGGUGUCGCg -3' miRNA: 3'- cAGGCGCCCGGg--CGGGcCUACAGCGa -5' |
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20419 | 5' | -65 | NC_004688.1 | + | 53653 | 0.68 | 0.337469 |
Target: 5'- -cCUGCGGGUCCGCCCaGGcuccggcauugacGUCGCc -3' miRNA: 3'- caGGCGCCCGGGCGGG-CCua-----------CAGCGa -5' |
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20419 | 5' | -65 | NC_004688.1 | + | 87919 | 0.69 | 0.299749 |
Target: 5'- -aCCGCaGGCCCGCUCcagGGAUGcgaCGCg -3' miRNA: 3'- caGGCGcCCGGGCGGG---CCUACa--GCGa -5' |
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20419 | 5' | -65 | NC_004688.1 | + | 61547 | 0.69 | 0.28663 |
Target: 5'- -aCCGCGGuacGCCCGCaUCGGGUGcUGCa -3' miRNA: 3'- caGGCGCC---CGGGCG-GGCCUACaGCGa -5' |
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20419 | 5' | -65 | NC_004688.1 | + | 27546 | 0.71 | 0.222491 |
Target: 5'- gGUCCaGCGGGCCCGgCCGGGgaucaggaaguUGacCGCg -3' miRNA: 3'- -CAGG-CGCCCGGGCgGGCCU-----------ACa-GCGa -5' |
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20419 | 5' | -65 | NC_004688.1 | + | 9821 | 1.06 | 0.000521 |
Target: 5'- cGUCCGCGGGCCCGCCCGGAUGUCGCUc -3' miRNA: 3'- -CAGGCGCCCGGGCGGGCCUACAGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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