Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20420 | 3' | -56.5 | NC_004688.1 | + | 20484 | 0.66 | 0.86724 |
Target: 5'- gCGCaCGGCGCGcuu-GCucuGGGCGAUCa -3' miRNA: 3'- gGCGaGCCGUGCuaguCG---CCUGCUAG- -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 58701 | 0.66 | 0.86724 |
Target: 5'- aUGCUgGGUGCGcuauuccUCGGCGGAgGGUUu -3' miRNA: 3'- gGCGAgCCGUGCu------AGUCGCCUgCUAG- -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 46251 | 0.66 | 0.866489 |
Target: 5'- cUCGUucgacauUCGGgGCGAUaaguggCGGCaGGACGGUCu -3' miRNA: 3'- -GGCG-------AGCCgUGCUA------GUCG-CCUGCUAG- -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 14036 | 0.66 | 0.859628 |
Target: 5'- cCCGCcgagcaagacgaUCGGCACGcccuucucGUCGGCGGuuuCcAUCg -3' miRNA: 3'- -GGCG------------AGCCGUGC--------UAGUCGCCu--GcUAG- -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 93203 | 0.66 | 0.851807 |
Target: 5'- -gGCUCGGCgggcuugccACGAU-GGCGGACcuuGUCa -3' miRNA: 3'- ggCGAGCCG---------UGCUAgUCGCCUGc--UAG- -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 39187 | 0.66 | 0.843785 |
Target: 5'- aCCGC-CGGUuuGGUCGGCGG-CGc-- -3' miRNA: 3'- -GGCGaGCCGugCUAGUCGCCuGCuag -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 73348 | 0.66 | 0.843785 |
Target: 5'- gCGCUCGGC-Ca---AGUGGACGAc- -3' miRNA: 3'- gGCGAGCCGuGcuagUCGCCUGCUag -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 104154 | 0.66 | 0.843785 |
Target: 5'- -gGCUCacgcGCGCGGU-AGCGGACGcaAUCg -3' miRNA: 3'- ggCGAGc---CGUGCUAgUCGCCUGC--UAG- -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 52333 | 0.66 | 0.827163 |
Target: 5'- gCGCgggCGGCGCaGAuUCAuCGGAgCGGUCg -3' miRNA: 3'- gGCGa--GCCGUG-CU-AGUcGCCU-GCUAG- -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 102912 | 0.67 | 0.818579 |
Target: 5'- aUCGCg-GGCGCGAcuggauaAGCGGGCGGc- -3' miRNA: 3'- -GGCGagCCGUGCUag-----UCGCCUGCUag -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 4970 | 0.67 | 0.809823 |
Target: 5'- gCGUUCGuaGCGaAUCgGGUGGGCGAUg -3' miRNA: 3'- gGCGAGCcgUGC-UAG-UCGCCUGCUAg -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 77233 | 0.67 | 0.786315 |
Target: 5'- aCGCUCcggcuucagcucgugGGCGCGGUau-CGGACGAUg -3' miRNA: 3'- gGCGAG---------------CCGUGCUAgucGCCUGCUAg -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 96715 | 0.67 | 0.773254 |
Target: 5'- aCGcCUCGGcCGCGAUaggcaccccCGGCaGGGCGAUg -3' miRNA: 3'- gGC-GAGCC-GUGCUA---------GUCG-CCUGCUAg -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 31033 | 0.68 | 0.767578 |
Target: 5'- cCCGCcgaGGCugGcgagguugccaggucGUCGGCGuuGGCGAUCa -3' miRNA: 3'- -GGCGag-CCGugC---------------UAGUCGC--CUGCUAG- -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 19556 | 0.68 | 0.763769 |
Target: 5'- gCCGCUcaacguuuaagCGGCgACGAUCcucauGUGGACG-UCg -3' miRNA: 3'- -GGCGA-----------GCCG-UGCUAGu----CGCCUGCuAG- -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 2405 | 0.68 | 0.762814 |
Target: 5'- gCCaCUCGGCgcuucucucuGCGAUCGGCGGGucuucguagauuuCGAUg -3' miRNA: 3'- -GGcGAGCCG----------UGCUAGUCGCCU-------------GCUAg -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 46114 | 0.68 | 0.744455 |
Target: 5'- --aCUCGGC-CGuuGUCgAGUGGACGAUCc -3' miRNA: 3'- ggcGAGCCGuGC--UAG-UCGCCUGCUAG- -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 104867 | 0.68 | 0.724743 |
Target: 5'- aCCGCgCGGCGaugaGAUCu-UGGAUGAUCu -3' miRNA: 3'- -GGCGaGCCGUg---CUAGucGCCUGCUAG- -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 105395 | 0.69 | 0.718763 |
Target: 5'- aCCGaugaugaccauugCGGCAagGGUCGGCGGACcGUCa -3' miRNA: 3'- -GGCga-----------GCCGUg-CUAGUCGCCUGcUAG- -5' |
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20420 | 3' | -56.5 | NC_004688.1 | + | 557 | 0.69 | 0.694592 |
Target: 5'- gCGC-CGGCACGGauggCAGauGACGAUg -3' miRNA: 3'- gGCGaGCCGUGCUa---GUCgcCUGCUAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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