Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20420 | 5' | -55.3 | NC_004688.1 | + | 73598 | 0.66 | 0.872363 |
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Target: 5'- uGGGAUCGaUGcGGGGCcGGGCCCG-CGg -3' miRNA: 3'- -UUCUAGC-GCaCCUUGcUCCGGGUuGC- -5' |
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| 20420 | 5' | -55.3 | NC_004688.1 | + | 86233 | 0.67 | 0.814476 |
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Target: 5'- gGAGcUCGuCGaGGGGC-AGGCCCAGCa -3' miRNA: 3'- -UUCuAGC-GCaCCUUGcUCCGGGUUGc -5' |
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| 20420 | 5' | -55.3 | NC_004688.1 | + | 48283 | 0.67 | 0.814476 |
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Target: 5'- -cGAUUGCGccGGAcaACGGGGaCCUGACGu -3' miRNA: 3'- uuCUAGCGCa-CCU--UGCUCC-GGGUUGC- -5' |
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| 20420 | 5' | -55.3 | NC_004688.1 | + | 79969 | 0.67 | 0.805438 |
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Target: 5'- cGGAUCGCcauUGcGGugGAGGgCCAugGg -3' miRNA: 3'- uUCUAGCGc--AC-CUugCUCCgGGUugC- -5' |
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| 20420 | 5' | -55.3 | NC_004688.1 | + | 17295 | 0.68 | 0.777364 |
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Target: 5'- gGGGAUUGCGcGGAAC-AGGCagcgCAGCGg -3' miRNA: 3'- -UUCUAGCGCaCCUUGcUCCGg---GUUGC- -5' |
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| 20420 | 5' | -55.3 | NC_004688.1 | + | 36840 | 0.7 | 0.665872 |
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Target: 5'- -uGGUCGCGUucaGAuACGAGGCCgCGACa -3' miRNA: 3'- uuCUAGCGCAc--CU-UGCUCCGG-GUUGc -5' |
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| 20420 | 5' | -55.3 | NC_004688.1 | + | 76379 | 0.72 | 0.53019 |
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Target: 5'- gAGGGUCcagaGCG-GGAACggGAGGCCCAGCc -3' miRNA: 3'- -UUCUAG----CGCaCCUUG--CUCCGGGUUGc -5' |
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| 20420 | 5' | -55.3 | NC_004688.1 | + | 11047 | 1.07 | 0.002998 |
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Target: 5'- gAAGAUCGCGUGGAACGAGGCCCAACGa -3' miRNA: 3'- -UUCUAGCGCACCUUGCUCCGGGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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