Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20421 | 5' | -54.8 | NC_004688.1 | + | 7000 | 0.66 | 0.89586 |
Target: 5'- cGGGCGUGgGCuuGGCGUuGAUgGCGUg -3' miRNA: 3'- -CCUGCGCgCGucUUGCAcCUAgUGCA- -5' |
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20421 | 5' | -54.8 | NC_004688.1 | + | 106357 | 0.67 | 0.882026 |
Target: 5'- cGGACGCGUcCAGAcaucCGUGcAUCAUGg -3' miRNA: 3'- -CCUGCGCGcGUCUu---GCACcUAGUGCa -5' |
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20421 | 5' | -54.8 | NC_004688.1 | + | 10981 | 0.67 | 0.867271 |
Target: 5'- cGAgGUGUGCAc-ACGcUGGAUCGCGUc -3' miRNA: 3'- cCUgCGCGCGUcuUGC-ACCUAGUGCA- -5' |
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20421 | 5' | -54.8 | NC_004688.1 | + | 35691 | 0.67 | 0.851637 |
Target: 5'- --cCGUGCGC-GAACGUGGGgguuccCACGg -3' miRNA: 3'- ccuGCGCGCGuCUUGCACCUa-----GUGCa -5' |
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20421 | 5' | -54.8 | NC_004688.1 | + | 17852 | 0.67 | 0.851637 |
Target: 5'- aGGGCGCGUGCGGucucaGGGUCAUc- -3' miRNA: 3'- -CCUGCGCGCGUCuugcaCCUAGUGca -5' |
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20421 | 5' | -54.8 | NC_004688.1 | + | 10656 | 0.67 | 0.851637 |
Target: 5'- gGGGCGgucaGCGCGGcguuguccgcGGCcUGGAUCGCGg -3' miRNA: 3'- -CCUGCg---CGCGUC----------UUGcACCUAGUGCa -5' |
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20421 | 5' | -54.8 | NC_004688.1 | + | 110697 | 0.68 | 0.817951 |
Target: 5'- cGGGCGCGCGUGGcAUG-GcGAUUGCGa -3' miRNA: 3'- -CCUGCGCGCGUCuUGCaC-CUAGUGCa -5' |
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20421 | 5' | -54.8 | NC_004688.1 | + | 31504 | 0.69 | 0.780521 |
Target: 5'- cGGCGCaaccuccGCGCAGAACGUu-GUCGCGg -3' miRNA: 3'- cCUGCG-------CGCGUCUUGCAccUAGUGCa -5' |
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20421 | 5' | -54.8 | NC_004688.1 | + | 47499 | 0.7 | 0.71263 |
Target: 5'- uGAUGCucGUGUAGAgGCGUGGAUCGCc- -3' miRNA: 3'- cCUGCG--CGCGUCU-UGCACCUAGUGca -5' |
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20421 | 5' | -54.8 | NC_004688.1 | + | 61211 | 0.73 | 0.557331 |
Target: 5'- gGGGCGuCGCGguGGAUcugGUGGAUCgGCGa -3' miRNA: 3'- -CCUGC-GCGCguCUUG---CACCUAG-UGCa -5' |
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20421 | 5' | -54.8 | NC_004688.1 | + | 29541 | 0.77 | 0.353828 |
Target: 5'- uGGGCGUGCGCAGAGUGcGGGcCACGUc -3' miRNA: 3'- -CCUGCGCGCGUCUUGCaCCUaGUGCA- -5' |
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20421 | 5' | -54.8 | NC_004688.1 | + | 11236 | 1.09 | 0.002801 |
Target: 5'- cGGACGCGCGCAGAACGUGGAUCACGUc -3' miRNA: 3'- -CCUGCGCGCGUCUUGCACCUAGUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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