Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20422 | 3' | -62.4 | NC_004688.1 | + | 39709 | 0.66 | 0.559409 |
Target: 5'- -uGGGCCUCCUguUG-GGGgUGGGcCGUCc -3' miRNA: 3'- guUCCGGAGGG--GCaCCCgAUCC-GCGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 41399 | 0.66 | 0.54957 |
Target: 5'- ----cCCUCCguacguCCGUGGGCgacAGcGCGCCg -3' miRNA: 3'- guuccGGAGG------GGCACCCGa--UC-CGCGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 24186 | 0.66 | 0.54957 |
Target: 5'- cCGGGGCCcCgCCCGUGGGgauCUcGGGaggaaGCCc -3' miRNA: 3'- -GUUCCGGaG-GGGCACCC---GA-UCCg----CGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 18218 | 0.66 | 0.539786 |
Target: 5'- -cAGGCCggugauggggCCCGUGGcGCc-GGUGCCg -3' miRNA: 3'- guUCCGGag--------GGGCACC-CGauCCGCGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 65564 | 0.66 | 0.530061 |
Target: 5'- --cGGCCaguaaUCCCCGaGGuGCaGGGUGUCa -3' miRNA: 3'- guuCCGG-----AGGGGCaCC-CGaUCCGCGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 16947 | 0.66 | 0.530061 |
Target: 5'- uCGGGGCCg-CUgGcGGGCUucaacacGGCGCCg -3' miRNA: 3'- -GUUCCGGagGGgCaCCCGAu------CCGCGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 67274 | 0.66 | 0.530061 |
Target: 5'- aGAGGCUgagCUcgaCCG-GGGagaagaUGGGCGCCg -3' miRNA: 3'- gUUCCGGa--GG---GGCaCCCg-----AUCCGCGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 39665 | 0.66 | 0.520401 |
Target: 5'- -cGGGCCUucgCCCCGccGGGC--GGUGUCu -3' miRNA: 3'- guUCCGGA---GGGGCa-CCCGauCCGCGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 43737 | 0.66 | 0.520401 |
Target: 5'- -cGGGCCUgccugcguagCCCGcGGGCcagGGGCGCa -3' miRNA: 3'- guUCCGGAg---------GGGCaCCCGa--UCCGCGg -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 27016 | 0.66 | 0.520401 |
Target: 5'- gAGGGCUauUCCcuCCGUgaccccGGGCcacGGCGCCa -3' miRNA: 3'- gUUCCGG--AGG--GGCA------CCCGau-CCGCGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 86936 | 0.67 | 0.473236 |
Target: 5'- uCGAGGCCgUCCCaCG-GGGCgcucaguucAGGUGaCCc -3' miRNA: 3'- -GUUCCGG-AGGG-GCaCCCGa--------UCCGC-GG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 109219 | 0.67 | 0.473236 |
Target: 5'- -cAGGCCUCacgaucaCGUcGGGC-GGGCGCa -3' miRNA: 3'- guUCCGGAGgg-----GCA-CCCGaUCCGCGg -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 95546 | 0.67 | 0.464059 |
Target: 5'- --cGGCCUCCgaaGcuUGGGCUGGGUuccuaggcgGCCa -3' miRNA: 3'- guuCCGGAGGgg-C--ACCCGAUCCG---------CGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 77227 | 0.67 | 0.461324 |
Target: 5'- --cGGCUUCagCUCGUGGGCgcgGuaucggacgauggcGGCGCCg -3' miRNA: 3'- guuCCGGAG--GGGCACCCGa--U--------------CCGCGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 92364 | 0.68 | 0.437103 |
Target: 5'- ---cGCC-CUCCGUGGGCgGGGCGg- -3' miRNA: 3'- guucCGGaGGGGCACCCGaUCCGCgg -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 77452 | 0.68 | 0.437103 |
Target: 5'- uCAccGCCagCCCCGUGGGCUcaaaGCCg -3' miRNA: 3'- -GUucCGGa-GGGGCACCCGAuccgCGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 25029 | 0.68 | 0.41964 |
Target: 5'- gAAGGCCUCCaCCaG-GGGCaUAGGacaccuGCCc -3' miRNA: 3'- gUUCCGGAGG-GG-CaCCCG-AUCCg-----CGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 109441 | 0.68 | 0.394256 |
Target: 5'- aUAAGGUcggCUCgCCCGUGGGCgucgauCGCCg -3' miRNA: 3'- -GUUCCG---GAG-GGGCACCCGaucc--GCGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 26306 | 0.69 | 0.346571 |
Target: 5'- cCGAGGCCgccgCCCUG-GGGCUGccaGCCu -3' miRNA: 3'- -GUUCCGGa---GGGGCaCCCGAUccgCGG- -5' |
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20422 | 3' | -62.4 | NC_004688.1 | + | 3380 | 0.7 | 0.339036 |
Target: 5'- uGAGGaa-CUCCGacaUGGGCUggAGGCGCCg -3' miRNA: 3'- gUUCCggaGGGGC---ACCCGA--UCCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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