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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20423 | 3' | -55.7 | NC_004688.1 | + | 91906 | 0.66 | 0.848857 |
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Target: 5'- ---cCCCCGGGCaacgcGAUCaGGCGAa--- -3' miRNA: 3'- caauGGGGCCCGa----CUAG-CCGCUauga -5' |
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| 20423 | 3' | -55.7 | NC_004688.1 | + | 88235 | 0.66 | 0.832164 |
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Target: 5'- gGUUACgaggucggCCGGGCUGA-CGGUGAggGCg -3' miRNA: 3'- -CAAUGg-------GGCCCGACUaGCCGCUa-UGa -5' |
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| 20423 | 3' | -55.7 | NC_004688.1 | + | 84584 | 0.67 | 0.814706 |
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Target: 5'- -aUACCCCuccGGGaugaGGUCGGCGAU-CUu -3' miRNA: 3'- caAUGGGG---CCCga--CUAGCCGCUAuGA- -5' |
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| 20423 | 3' | -55.7 | NC_004688.1 | + | 33546 | 0.7 | 0.656356 |
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Target: 5'- ---uUCCCGGGCUGAcCGGCcugaccguuGAUACc -3' miRNA: 3'- caauGGGGCCCGACUaGCCG---------CUAUGa -5' |
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| 20423 | 3' | -55.7 | NC_004688.1 | + | 100578 | 0.7 | 0.614413 |
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Target: 5'- --cGCCCUgggGGGCUucuuugCGGCGAUACUg -3' miRNA: 3'- caaUGGGG---CCCGAcua---GCCGCUAUGA- -5' |
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| 20423 | 3' | -55.7 | NC_004688.1 | + | 39191 | 0.76 | 0.317908 |
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Target: 5'- gGUUACCgCCGGuuUGGUCGGCGGcGCUg -3' miRNA: 3'- -CAAUGG-GGCCcgACUAGCCGCUaUGA- -5' |
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| 20423 | 3' | -55.7 | NC_004688.1 | + | 14224 | 1.08 | 0.002318 |
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Target: 5'- cGUUACCCCGGGCUGAUCGGCGAUACUg -3' miRNA: 3'- -CAAUGGGGCCCGACUAGCCGCUAUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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