miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20424 3' -59.5 NC_004688.1 + 79847 0.67 0.572165
Target:  5'- ---cGGGCgcCCGuCGGCCGaGUGGGCCc -3'
miRNA:   3'- uucuUCCGa-GGC-GCCGGC-CACUUGGa -5'
20424 3' -59.5 NC_004688.1 + 44780 0.68 0.551847
Target:  5'- gAGGAAGGUgcccgCCGCGGU-GGUG-GCCg -3'
miRNA:   3'- -UUCUUCCGa----GGCGCCGgCCACuUGGa -5'
20424 3' -59.5 NC_004688.1 + 42328 0.7 0.42708
Target:  5'- -cGAGGGC---GCGGUCGGUGAACUg -3'
miRNA:   3'- uuCUUCCGaggCGCCGGCCACUUGGa -5'
20424 3' -59.5 NC_004688.1 + 14722 1.05 0.00144
Target:  5'- cAAGAAGGCUCCGCGGCCGGUGAACCUa -3'
miRNA:   3'- -UUCUUCCGAGGCGCCGGCCACUUGGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.