Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20424 | 5' | -55 | NC_004688.1 | + | 82437 | 0.65 | 0.90312 |
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Target: 5'- gGuGGUGCUCcaguugucauugaGGGCCGGGAuGUCgCCAc -3' miRNA: 3'- aCuCUAUGAG-------------CCUGGUCCU-CAG-GGUc -5' |
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| 20424 | 5' | -55 | NC_004688.1 | + | 66139 | 0.66 | 0.868564 |
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Target: 5'- -cAGggGCUUGGGCgAGGugcGGUCCCAc -3' miRNA: 3'- acUCuaUGAGCCUGgUCC---UCAGGGUc -5' |
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| 20424 | 5' | -55 | NC_004688.1 | + | 71571 | 0.67 | 0.844715 |
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Target: 5'- -cAGAUAC-CGGGCgAGGGGUgugCCCAu -3' miRNA: 3'- acUCUAUGaGCCUGgUCCUCA---GGGUc -5' |
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| 20424 | 5' | -55 | NC_004688.1 | + | 89335 | 0.67 | 0.826919 |
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Target: 5'- gUGAG-UGCagUCGG-CCAGGAccuugauGUCCCGGc -3' miRNA: 3'- -ACUCuAUG--AGCCuGGUCCU-------CAGGGUC- -5' |
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| 20424 | 5' | -55 | NC_004688.1 | + | 41776 | 0.68 | 0.786113 |
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Target: 5'- cGGGAUcggGCugccgucuucgacaaUCGGGCCAgGGAGguaugCCCAGg -3' miRNA: 3'- aCUCUA---UG---------------AGCCUGGU-CCUCa----GGGUC- -5' |
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| 20424 | 5' | -55 | NC_004688.1 | + | 42221 | 0.69 | 0.733327 |
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Target: 5'- cUGAcGUAUUCGGACgAGaucAGUCCCGGa -3' miRNA: 3'- -ACUcUAUGAGCCUGgUCc--UCAGGGUC- -5' |
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| 20424 | 5' | -55 | NC_004688.1 | + | 100120 | 0.7 | 0.713007 |
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Target: 5'- cGAGAUcacACUgGGGCCGGGuGUgcgcaUCCAGa -3' miRNA: 3'- aCUCUA---UGAgCCUGGUCCuCA-----GGGUC- -5' |
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| 20424 | 5' | -55 | NC_004688.1 | + | 91454 | 0.71 | 0.620134 |
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Target: 5'- -cAGAUACUCGGACUcGGGGcgguggaacuucacgCCCAGg -3' miRNA: 3'- acUCUAUGAGCCUGGuCCUCa--------------GGGUC- -5' |
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| 20424 | 5' | -55 | NC_004688.1 | + | 14687 | 1.08 | 0.002751 |
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Target: 5'- uUGAGAUACUCGGACCAGGAGUCCCAGu -3' miRNA: 3'- -ACUCUAUGAGCCUGGUCCUCAGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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