miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20425 3' -60.5 NC_004688.1 + 95212 0.66 0.596491
Target:  5'- gGAugCUgG-GAgUGGGCCUcGCgGCGCa -3'
miRNA:   3'- -UUugGAgCgCUgACCCGGA-CGgCGUG- -5'
20425 3' -60.5 NC_004688.1 + 4651 0.66 0.59341
Target:  5'- aGAGCCUCGUGcaggcacaauuccuGCcacGGGUCgagaaugagGCCGCACg -3'
miRNA:   3'- -UUUGGAGCGC--------------UGa--CCCGGa--------CGGCGUG- -5'
20425 3' -60.5 NC_004688.1 + 42629 0.66 0.58623
Target:  5'- aGAGCCUCGgGAUUGcccaucauGGCCUGaCGCuCg -3'
miRNA:   3'- -UUUGGAGCgCUGAC--------CCGGACgGCGuG- -5'
20425 3' -60.5 NC_004688.1 + 52829 0.66 0.576002
Target:  5'- cAGGCC-CgGCGACUGGcccaaugcuuGCUgGCCGCGCu -3'
miRNA:   3'- -UUUGGaG-CGCUGACC----------CGGaCGGCGUG- -5'
20425 3' -60.5 NC_004688.1 + 18116 0.67 0.545589
Target:  5'- gAAGCCgugggugagCGUGACcucaaUGGGCCcGCgGCACc -3'
miRNA:   3'- -UUUGGa--------GCGCUG-----ACCCGGaCGgCGUG- -5'
20425 3' -60.5 NC_004688.1 + 70366 0.67 0.542574
Target:  5'- -cGCCUCGaCGACcucggUGaGGCCgaaguucucauagcUGCCGUACa -3'
miRNA:   3'- uuUGGAGC-GCUG-----AC-CCGG--------------ACGGCGUG- -5'
20425 3' -60.5 NC_004688.1 + 48857 0.67 0.535563
Target:  5'- uAAACgUCGCGGCUGcuagccGCCUGCauCGCAa -3'
miRNA:   3'- -UUUGgAGCGCUGACc-----CGGACG--GCGUg -5'
20425 3' -60.5 NC_004688.1 + 9680 0.7 0.36237
Target:  5'- aGAGCCUCGCGAa--GGCUgaUGCCGCc- -3'
miRNA:   3'- -UUUGGAGCGCUgacCCGG--ACGGCGug -5'
20425 3' -60.5 NC_004688.1 + 57930 0.72 0.274827
Target:  5'- -cACCUCGgGGCUGcuGGCCUGUgGCGg -3'
miRNA:   3'- uuUGGAGCgCUGAC--CCGGACGgCGUg -5'
20425 3' -60.5 NC_004688.1 + 14817 1.07 0.000838
Target:  5'- uAAACCUCGCGACUGGGCCUGCCGCACg -3'
miRNA:   3'- -UUUGGAGCGCUGACCCGGACGGCGUG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.