Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20425 | 5' | -49.2 | NC_004688.1 | + | 51034 | 0.66 | 0.99455 |
Target: 5'- --cAGCUGCcCGCUggguGUCGggaugGAGCGa -3' miRNA: 3'- uuuUCGAUGuGCGGau--CAGCa----CUUGC- -5' |
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20425 | 5' | -49.2 | NC_004688.1 | + | 868 | 0.66 | 0.99455 |
Target: 5'- --cGGUUGCACGCCg---CGUGGAg- -3' miRNA: 3'- uuuUCGAUGUGCGGaucaGCACUUgc -5' |
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20425 | 5' | -49.2 | NC_004688.1 | + | 108570 | 0.69 | 0.963821 |
Target: 5'- cAAGGGUUACucgaccGCGCCgAGUCG-GAGCGc -3' miRNA: 3'- -UUUUCGAUG------UGCGGaUCAGCaCUUGC- -5' |
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20425 | 5' | -49.2 | NC_004688.1 | + | 76397 | 0.69 | 0.956083 |
Target: 5'- gAAGGGCUuacACGCGCUgagGGUCcaGAGCGg -3' miRNA: 3'- -UUUUCGA---UGUGCGGa--UCAGcaCUUGC- -5' |
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20425 | 5' | -49.2 | NC_004688.1 | + | 79164 | 0.76 | 0.685938 |
Target: 5'- --cAGCcacugGCGCGCCUGGUUGUaGAGCGc -3' miRNA: 3'- uuuUCGa----UGUGCGGAUCAGCA-CUUGC- -5' |
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20425 | 5' | -49.2 | NC_004688.1 | + | 14780 | 1.08 | 0.009361 |
Target: 5'- cAAAAGCUACACGCCUAGUCGUGAACGg -3' miRNA: 3'- -UUUUCGAUGUGCGGAUCAGCACUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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