Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20428 | 5' | -58 | NC_004688.1 | + | 83159 | 0.66 | 0.69262 |
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Target: 5'- uGGGGgUGUugCGCGGGUGauGCAcCCAc -3' miRNA: 3'- -UUUCgACAugGCGCCCGC--CGUuGGUu -5' |
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| 20428 | 5' | -58 | NC_004688.1 | + | 46497 | 0.66 | 0.69262 |
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Target: 5'- uAAGCcGaucGCCGCGGGCuccccGGUGACCAc -3' miRNA: 3'- uUUCGaCa--UGGCGCCCG-----CCGUUGGUu -5' |
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| 20428 | 5' | -58 | NC_004688.1 | + | 3715 | 0.67 | 0.65051 |
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Target: 5'- --cGCUGcugGuuGCaGGCGGCAugCAAa -3' miRNA: 3'- uuuCGACa--UggCGcCCGCCGUugGUU- -5' |
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| 20428 | 5' | -58 | NC_004688.1 | + | 65465 | 0.67 | 0.639919 |
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Target: 5'- cAGGCcGUGCCaCGGGUGGCccuCCGGg -3' miRNA: 3'- uUUCGaCAUGGcGCCCGCCGuu-GGUU- -5' |
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| 20428 | 5' | -58 | NC_004688.1 | + | 63888 | 0.67 | 0.629322 |
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Target: 5'- cAAGGCcg-ACCGCGgugaaugcGGUGGCAACCGc -3' miRNA: 3'- -UUUCGacaUGGCGC--------CCGCCGUUGGUu -5' |
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| 20428 | 5' | -58 | NC_004688.1 | + | 20584 | 0.68 | 0.539134 |
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Target: 5'- cGAGGCUGUGCagcggcuugucguagCGCaGGGCGuCGACCAGc -3' miRNA: 3'- -UUUCGACAUG---------------GCG-CCCGCcGUUGGUU- -5' |
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| 20428 | 5' | -58 | NC_004688.1 | + | 102908 | 0.69 | 0.524804 |
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Target: 5'- cGGGCgcgACUggauaaGCGGGCGGCGACCu- -3' miRNA: 3'- uUUCGacaUGG------CGCCCGCCGUUGGuu -5' |
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| 20428 | 5' | -58 | NC_004688.1 | + | 15811 | 1.04 | 0.002145 |
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Target: 5'- cAAAGCUGUACCGCGGGCGGCAACCAAa -3' miRNA: 3'- -UUUCGACAUGGCGCCCGCCGUUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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