Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2043 | 3' | -50.5 | NC_001347.2 | + | 182187 | 0.66 | 0.998942 |
Target: 5'- -uGUGGGCCGgcccUCGGggugcgggUGGCGGGCUg -3' miRNA: 3'- guCACCUGGC----AGUUaaa-----GCUGCCUGG- -5' |
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2043 | 3' | -50.5 | NC_001347.2 | + | 11834 | 0.66 | 0.999127 |
Target: 5'- ---gGGGCCGUUg--UUCGGaGGACCc -3' miRNA: 3'- gucaCCUGGCAGuuaAAGCUgCCUGG- -5' |
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2043 | 3' | -50.5 | NC_001347.2 | + | 173106 | 0.66 | 0.999416 |
Target: 5'- -cGUGGAUCua-GAUUUCGGCGugguGGCCg -3' miRNA: 3'- guCACCUGGcagUUAAAGCUGC----CUGG- -5' |
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2043 | 3' | -50.5 | NC_001347.2 | + | 90784 | 0.66 | 0.999526 |
Target: 5'- gGGUGGAuccuCCGUCGuccUCG-CGGccGCCa -3' miRNA: 3'- gUCACCU----GGCAGUuaaAGCuGCC--UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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