miRNA: 3'- guCACCuGGCAGUUaaaGCUGC-CUGg -5'
miRNA: 3'- gUCA-CCUGGCAGUuaaag----CugCCUGG- -5'
miRNA: 3'- guCACCuGGCAGUUaaAGCUGCCuGG- -5'
miRNA: 3'- -GUCaCCUGGCAGUUAA----------AGCUGCCU---GG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2043 | 3' | -50.5 | NC_001347.2 | + | 128450 | 0.7 | 0.980466 |
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Target: 5'- -cGUGG-CCGUCAAg--CGGCGcGACg -3' miRNA: 3'- guCACCuGGCAGUUaaaGCUGC-CUGg -5' |
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| 2043 | 3' | -50.5 | NC_001347.2 | + | 31681 | 0.71 | 0.974835 |
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Target: 5'- uGGUcGGGCCGUCGcggacgaaaGugGGGCCg -3' miRNA: 3'- gUCA-CCUGGCAGUuaaag----CugCCUGG- -5' |
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| 2043 | 3' | -50.5 | NC_001347.2 | + | 8676 | 0.75 | 0.889088 |
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Target: 5'- -cGUGGuCCGUCGAaaUUGACGGuCCa -3' miRNA: 3'- guCACCuGGCAGUUaaAGCUGCCuGG- -5' |
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| 2043 | 3' | -50.5 | NC_001347.2 | + | 209961 | 0.78 | 0.746595 |
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Target: 5'- cCAGcGGACCGUCAGUUguuggcguagUUGACGGAgucCCg -3' miRNA: 3'- -GUCaCCUGGCAGUUAA----------AGCUGCCU---GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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