Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20430 | 3' | -63.7 | NC_004688.1 | + | 75467 | 0.67 | 0.440893 |
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Target: 5'- uCCAGuauucguucgucCCGGaaaGGUGGGGCCCGUcgaauccgguaauGGCGa -3' miRNA: 3'- -GGUCc-----------GGCCa--CUACCCCGGGCA-------------CCGC- -5' |
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| 20430 | 3' | -63.7 | NC_004688.1 | + | 37307 | 0.67 | 0.417748 |
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Target: 5'- aCCucgucGCCGGUGuUGGuGCCCGUGGa- -3' miRNA: 3'- -GGuc---CGGCCACuACCcCGGGCACCgc -5' |
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| 20430 | 3' | -63.7 | NC_004688.1 | + | 24200 | 0.68 | 0.392918 |
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Target: 5'- aCCGuuGCCGGUGcccgGGGccccGCCCGUGGgGa -3' miRNA: 3'- -GGUc-CGGCCACua--CCC----CGGGCACCgC- -5' |
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| 20430 | 3' | -63.7 | NC_004688.1 | + | 45091 | 0.69 | 0.353727 |
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Target: 5'- gCucGUCGGUGGUGGcGGCCUgcuuagcguuGUGGCGc -3' miRNA: 3'- gGucCGGCCACUACC-CCGGG----------CACCGC- -5' |
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| 20430 | 3' | -63.7 | NC_004688.1 | + | 70020 | 0.75 | 0.14812 |
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Target: 5'- uCCAGGUCGGUGAacaugauguaggcGGGGCuauccCCGUGGCc -3' miRNA: 3'- -GGUCCGGCCACUa------------CCCCG-----GGCACCGc -5' |
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| 20430 | 3' | -63.7 | NC_004688.1 | + | 18220 | 1.08 | 0.000511 |
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Target: 5'- uCCAGGCCGGUGAUGGGGCCCGUGGCGc -3' miRNA: 3'- -GGUCCGGCCACUACCCCGGGCACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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