Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20431 | 3' | -55.9 | NC_004688.1 | + | 24490 | 0.66 | 0.883053 |
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Target: 5'- --gAGAacGGuuucccguccguGCCGuACUCCAGC-UGGGGg -3' miRNA: 3'- ccaUCU--CC------------UGGC-UGAGGUCGuACCCC- -5' |
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| 20431 | 3' | -55.9 | NC_004688.1 | + | 26047 | 0.66 | 0.86775 |
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Target: 5'- ---cGGGGGCaCGGCUCCGGggaggccCGUGGGc -3' miRNA: 3'- ccauCUCCUG-GCUGAGGUC-------GUACCCc -5' |
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| 20431 | 3' | -55.9 | NC_004688.1 | + | 82435 | 0.66 | 0.866242 |
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Target: 5'- uGGUGcuccaguugucauuGAGGGCCGggaugucgccACUCCAGaugucGGGGa -3' miRNA: 3'- -CCAU--------------CUCCUGGC----------UGAGGUCgua--CCCC- -5' |
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| 20431 | 3' | -55.9 | NC_004688.1 | + | 12659 | 0.67 | 0.836848 |
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Target: 5'- uGGgcGAccucGGACuuCGGCaCCAGCAUGGGc -3' miRNA: 3'- -CCauCU----CCUG--GCUGaGGUCGUACCCc -5' |
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| 20431 | 3' | -55.9 | NC_004688.1 | + | 98816 | 0.69 | 0.745619 |
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Target: 5'- --aAGAGGACUGcCUagUCAGCggGGGGc -3' miRNA: 3'- ccaUCUCCUGGCuGA--GGUCGuaCCCC- -5' |
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| 20431 | 3' | -55.9 | NC_004688.1 | + | 49511 | 0.69 | 0.725859 |
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Target: 5'- gGGUAGGGcGAUUGACgCUGGCgaacauGUGGGGu -3' miRNA: 3'- -CCAUCUC-CUGGCUGaGGUCG------UACCCC- -5' |
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| 20431 | 3' | -55.9 | NC_004688.1 | + | 78906 | 0.72 | 0.572165 |
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Target: 5'- aGGUAGAGGAUCGGCuugUCUgacggAGCcacGGGGa -3' miRNA: 3'- -CCAUCUCCUGGCUG---AGG-----UCGua-CCCC- -5' |
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| 20431 | 3' | -55.9 | NC_004688.1 | + | 6521 | 0.73 | 0.511927 |
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Target: 5'- cGGUAGAGGAUCagGGCgcgagcgCCGGCGaggucugggcUGGGGu -3' miRNA: 3'- -CCAUCUCCUGG--CUGa------GGUCGU----------ACCCC- -5' |
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| 20431 | 3' | -55.9 | NC_004688.1 | + | 18310 | 1.12 | 0.001523 |
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Target: 5'- uGGUAGAGGACCGACUCCAGCAUGGGGu -3' miRNA: 3'- -CCAUCUCCUGGCUGAGGUCGUACCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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