Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20433 | 3' | -50.6 | NC_004688.1 | + | 60916 | 0.66 | 0.985802 |
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Target: 5'- aUCUUcGAAAGGg--GCCGGCcaauGGUUCu -3' miRNA: 3'- -GGAA-CUUUCCgaaCGGCCGcu--UCAAG- -5' |
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| 20433 | 3' | -50.6 | NC_004688.1 | + | 39703 | 0.66 | 0.977169 |
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Target: 5'- uCCUguUGGGgguGGGCcguCCGGCGggGUUg -3' miRNA: 3'- -GGA--ACUU---UCCGaacGGCCGCuuCAAg -5' |
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| 20433 | 3' | -50.6 | NC_004688.1 | + | 101706 | 0.66 | 0.977169 |
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Target: 5'- aCUUUGGGuuGGUcaacgUGCCGGUGAAGa-- -3' miRNA: 3'- -GGAACUUu-CCGa----ACGGCCGCUUCaag -5' |
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| 20433 | 3' | -50.6 | NC_004688.1 | + | 6514 | 0.67 | 0.968524 |
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Target: 5'- ---gGAucAGGGCgcgagcGCCGGCGAGGUc- -3' miRNA: 3'- ggaaCU--UUCCGaa----CGGCCGCUUCAag -5' |
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| 20433 | 3' | -50.6 | NC_004688.1 | + | 62603 | 0.68 | 0.957774 |
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Target: 5'- gCCUUGc--GGCUaa-CGGCGAGGUUUc -3' miRNA: 3'- -GGAACuuuCCGAacgGCCGCUUCAAG- -5' |
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| 20433 | 3' | -50.6 | NC_004688.1 | + | 56482 | 0.7 | 0.876463 |
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Target: 5'- gCUgcagGGucAGGGCgcGCCGGCGAAGcUCu -3' miRNA: 3'- gGAa---CU--UUCCGaaCGGCCGCUUCaAG- -5' |
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| 20433 | 3' | -50.6 | NC_004688.1 | + | 25414 | 0.72 | 0.826397 |
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Target: 5'- gCCgcUGAAuccagcuacGGGCgUGCCGGUGggGUUg -3' miRNA: 3'- -GGa-ACUU---------UCCGaACGGCCGCuuCAAg -5' |
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| 20433 | 3' | -50.6 | NC_004688.1 | + | 102387 | 0.72 | 0.788918 |
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Target: 5'- aCCgUUGAGcAGGUgugGCCGGCGGAGc-- -3' miRNA: 3'- -GG-AACUU-UCCGaa-CGGCCGCUUCaag -5' |
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| 20433 | 3' | -50.6 | NC_004688.1 | + | 18645 | 1.14 | 0.003199 |
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Target: 5'- aCCUUGAAAGGCUUGCCGGCGAAGUUCg -3' miRNA: 3'- -GGAACUUUCCGAACGGCCGCUUCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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