Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20435 | 3' | -53.6 | NC_004688.1 | + | 104163 | 0.66 | 0.931246 |
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Target: 5'- -cUGAUGGGuGGCUcaCGCGCgCGGuaGCGg -3' miRNA: 3'- aaACUACUC-CUGAaaGUGCG-GCC--CGC- -5' |
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| 20435 | 3' | -53.6 | NC_004688.1 | + | 68164 | 0.66 | 0.919993 |
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Target: 5'- --cGGUGAGGAUUc-CGCGCuCGGGa- -3' miRNA: 3'- aaaCUACUCCUGAaaGUGCG-GCCCgc -5' |
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| 20435 | 3' | -53.6 | NC_004688.1 | + | 61226 | 0.66 | 0.913974 |
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Target: 5'- -aUGAUGAGGAa----GgGCgGGGCGu -3' miRNA: 3'- aaACUACUCCUgaaagUgCGgCCCGC- -5' |
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| 20435 | 3' | -53.6 | NC_004688.1 | + | 20007 | 0.67 | 0.894363 |
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Target: 5'- -aUGAUGGGGGCg--C-CGUCGGGg- -3' miRNA: 3'- aaACUACUCCUGaaaGuGCGGCCCgc -5' |
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| 20435 | 3' | -53.6 | NC_004688.1 | + | 39675 | 0.67 | 0.887318 |
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Target: 5'- gUUGGUuucucGGGCcUUCGCcccGCCGGGCGg -3' miRNA: 3'- aAACUAcu---CCUGaAAGUG---CGGCCCGC- -5' |
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| 20435 | 3' | -53.6 | NC_004688.1 | + | 54018 | 0.67 | 0.887318 |
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Target: 5'- --gGGUGAGGuag--CGCuGCCGGGUGu -3' miRNA: 3'- aaaCUACUCCugaaaGUG-CGGCCCGC- -5' |
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| 20435 | 3' | -53.6 | NC_004688.1 | + | 73831 | 0.67 | 0.872492 |
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Target: 5'- -----cGGGGGCUaauucccggaugUUgGCGCCGGGCc -3' miRNA: 3'- aaacuaCUCCUGA------------AAgUGCGGCCCGc -5' |
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| 20435 | 3' | -53.6 | NC_004688.1 | + | 18211 | 0.67 | 0.864722 |
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Target: 5'- -gUGAUGGGGcCcgUgGCGCCGGuGCc -3' miRNA: 3'- aaACUACUCCuGaaAgUGCGGCC-CGc -5' |
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| 20435 | 3' | -53.6 | NC_004688.1 | + | 49152 | 0.71 | 0.672692 |
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Target: 5'- -gUGAUGAGGuGCgc-CGCGCCGcGGCc -3' miRNA: 3'- aaACUACUCC-UGaaaGUGCGGC-CCGc -5' |
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| 20435 | 3' | -53.6 | NC_004688.1 | + | 19167 | 1.08 | 0.003686 |
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Target: 5'- aUUUGAUGAGGACUUUCACGCCGGGCGg -3' miRNA: 3'- -AAACUACUCCUGAAAGUGCGGCCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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