Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20435 | 5' | -51.4 | NC_004688.1 | + | 5554 | 0.66 | 0.968789 |
Target: 5'- --gACCU--GGCGCAuCAgcgCCUCGUUCu -3' miRNA: 3'- cagUGGAcaUCGCGU-GUa--GGAGUAAG- -5' |
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20435 | 5' | -51.4 | NC_004688.1 | + | 79153 | 0.66 | 0.965453 |
Target: 5'- -gCGCCUGguuguagAGCGCGCcgaCCUCGUc- -3' miRNA: 3'- caGUGGACa------UCGCGUGua-GGAGUAag -5' |
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20435 | 5' | -51.4 | NC_004688.1 | + | 37263 | 0.66 | 0.957649 |
Target: 5'- cGUCugCUcccGCGCAUAUCCaccacccguggcuUCAUUCg -3' miRNA: 3'- -CAGugGAcauCGCGUGUAGG-------------AGUAAG- -5' |
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20435 | 5' | -51.4 | NC_004688.1 | + | 77675 | 0.69 | 0.8984 |
Target: 5'- -gCGCUUGUcgcagcGCGCGCAUCCaaaugUCAUUCc -3' miRNA: 3'- caGUGGACAu-----CGCGUGUAGG-----AGUAAG- -5' |
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20435 | 5' | -51.4 | NC_004688.1 | + | 19127 | 1.11 | 0.003714 |
Target: 5'- gGUCACCUGUAGCGCACAUCCUCAUUCa -3' miRNA: 3'- -CAGUGGACAUCGCGUGUAGGAGUAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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