Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20436 | 3' | -49.3 | NC_004688.1 | + | 51316 | 0.66 | 0.994877 |
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Target: 5'- gAGGUuGAgCGUCugcgcaaauugguugGGGAGGAagcGUGAGCa -3' miRNA: 3'- gUCCAuUUgGCAG---------------CUCUUCU---CACUCG- -5' |
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| 20436 | 3' | -49.3 | NC_004688.1 | + | 91697 | 0.66 | 0.994549 |
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Target: 5'- cCAGGccGAACCGUUGucGAGcGUGgAGCa -3' miRNA: 3'- -GUCCa-UUUGGCAGCucUUCuCAC-UCG- -5' |
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| 20436 | 3' | -49.3 | NC_004688.1 | + | 89465 | 0.66 | 0.993653 |
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Target: 5'- -cGGUcguACCGUCGAGAAuauucagucGAG-GGGUg -3' miRNA: 3'- guCCAuu-UGGCAGCUCUU---------CUCaCUCG- -5' |
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| 20436 | 3' | -49.3 | NC_004688.1 | + | 47835 | 0.67 | 0.991509 |
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Target: 5'- cCGGGgcca-CGUCGAGAA-AGUuGAGCg -3' miRNA: 3'- -GUCCauuugGCAGCUCUUcUCA-CUCG- -5' |
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| 20436 | 3' | -49.3 | NC_004688.1 | + | 90939 | 0.67 | 0.991267 |
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Target: 5'- aCAGG---GCCGggagauccaaucCGGGGAGAGUGGGg -3' miRNA: 3'- -GUCCauuUGGCa-----------GCUCUUCUCACUCg -5' |
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| 20436 | 3' | -49.3 | NC_004688.1 | + | 79848 | 0.68 | 0.983632 |
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Target: 5'- aCGGGcgc-CCGUCG-GccGAGUGGGCc -3' miRNA: 3'- -GUCCauuuGGCAGCuCuuCUCACUCG- -5' |
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| 20436 | 3' | -49.3 | NC_004688.1 | + | 39695 | 0.69 | 0.964649 |
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Target: 5'- gGGGUGGGCCGUCcGGcGGGGUugguuucucGGGCc -3' miRNA: 3'- gUCCAUUUGGCAGcUCuUCUCA---------CUCG- -5' |
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| 20436 | 3' | -49.3 | NC_004688.1 | + | 40219 | 0.69 | 0.957156 |
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Target: 5'- uCGGGUAggUCGUCGAu--GAGUuuuucgacGAGCg -3' miRNA: 3'- -GUCCAUuuGGCAGCUcuuCUCA--------CUCG- -5' |
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| 20436 | 3' | -49.3 | NC_004688.1 | + | 7435 | 0.7 | 0.948647 |
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Target: 5'- -cGGUG--UCGgCGAGAAGGGUGAcGCa -3' miRNA: 3'- guCCAUuuGGCaGCUCUUCUCACU-CG- -5' |
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| 20436 | 3' | -49.3 | NC_004688.1 | + | 21851 | 1.13 | 0.005236 |
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Target: 5'- uCAGGUAAACCGUCGAGAAGAGUGAGCa -3' miRNA: 3'- -GUCCAUUUGGCAGCUCUUCUCACUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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