Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2045 | 3' | -59.3 | NC_001347.2 | + | 173656 | 0.74 | 0.39904 |
Target: 5'- uGGGAGGAGgUGgCGGCgguGGCGGCa -3' miRNA: 3'- -UCUUCCUCgAUgGCCGaguCCGCCGg -5' |
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2045 | 3' | -59.3 | NC_001347.2 | + | 36667 | 0.75 | 0.390954 |
Target: 5'- cGucGGAGCUcagACCgGGCUCcggcGGGUGGCCu -3' miRNA: 3'- uCuuCCUCGA---UGG-CCGAG----UCCGCCGG- -5' |
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2045 | 3' | -59.3 | NC_001347.2 | + | 113170 | 0.76 | 0.330232 |
Target: 5'- aAGAGGGAacgcgcgccccaGCcGCCGGCgu-GGCGGCCu -3' miRNA: 3'- -UCUUCCU------------CGaUGGCCGaguCCGCCGG- -5' |
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2045 | 3' | -59.3 | NC_001347.2 | + | 172534 | 0.77 | 0.295954 |
Target: 5'- --cGGGGGCccGCUGGCUCGGcGCGGCUg -3' miRNA: 3'- ucuUCCUCGa-UGGCCGAGUC-CGCCGG- -5' |
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2045 | 3' | -59.3 | NC_001347.2 | + | 87760 | 0.77 | 0.28944 |
Target: 5'- cGAAGGAGCc-CUGGCcCAGGCGGUa -3' miRNA: 3'- uCUUCCUCGauGGCCGaGUCCGCCGg -5' |
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2045 | 3' | -59.3 | NC_001347.2 | + | 126881 | 0.78 | 0.246974 |
Target: 5'- ---uGGGGCUgGCCGGCUacGGCGGCCg -3' miRNA: 3'- ucuuCCUCGA-UGGCCGAguCCGCCGG- -5' |
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2045 | 3' | -59.3 | NC_001347.2 | + | 156763 | 0.85 | 0.089549 |
Target: 5'- gAGGAGGAGUUGCUGGCUCuggcgggagaagggaAGGCGGCa -3' miRNA: 3'- -UCUUCCUCGAUGGCCGAG---------------UCCGCCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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