Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 20454 | 3' | -61.6 | NC_004688.1 | + | 9561 | 0.66 | 0.592787 |
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Target: 5'- uGCCGCC-CGGAggucuucuUCGCuCGACucuUCugCGg -3' miRNA: 3'- -CGGCGGcGCCU--------AGCG-GCUGu--GGugGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 5256 | 0.66 | 0.592787 |
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Target: 5'- cGCC-CCGCauuGGuGUCGCCGuCGCCcaGCCa -3' miRNA: 3'- -CGGcGGCG---CC-UAGCGGCuGUGG--UGGc -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 66189 | 0.66 | 0.602671 |
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Target: 5'- -aCGcCCGUGGGcUUGaCCGGCACCugCu -3' miRNA: 3'- cgGC-GGCGCCU-AGC-GGCUGUGGugGc -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 27909 | 0.66 | 0.609603 |
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Target: 5'- gGUgGCCGUGGuuccgggcgaacccGUCGCCGugGuCCugACCGu -3' miRNA: 3'- -CGgCGGCGCC--------------UAGCGGCugU-GG--UGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 16621 | 0.66 | 0.611585 |
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Target: 5'- cCCGCCGCGuuacaacuccacaGAuUUGCCGGgGCCAaauCCGa -3' miRNA: 3'- cGGCGGCGC-------------CU-AGCGGCUgUGGU---GGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 29501 | 0.66 | 0.612576 |
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Target: 5'- uCCGUCGCuccccGGGUCuGCCGACAaaacggUACCGa -3' miRNA: 3'- cGGCGGCG-----CCUAG-CGGCUGUg-----GUGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 27436 | 0.66 | 0.612576 |
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Target: 5'- cGUCGUCGCGauuGAauUCGUCGAuCGCCAUCu -3' miRNA: 3'- -CGGCGGCGC---CU--AGCGGCU-GUGGUGGc -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 49974 | 0.66 | 0.622494 |
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Target: 5'- aCgGCUGCGGGUCGgCGAaaucaaGCCcugguACCGg -3' miRNA: 3'- cGgCGGCGCCUAGCgGCUg-----UGG-----UGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 102454 | 0.66 | 0.642345 |
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Target: 5'- cGCauggGCCGCGaacGAUgagGCCGACGCUAUCGc -3' miRNA: 3'- -CGg---CGGCGC---CUAg--CGGCUGUGGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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