Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20454 | 3' | -61.6 | NC_004688.1 | + | 49974 | 0.66 | 0.622494 |
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Target: 5'- aCgGCUGCGGGUCGgCGAaaucaaGCCcugguACCGg -3' miRNA: 3'- cGgCGGCGCCUAGCgGCUg-----UGG-----UGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 30807 | 0.66 | 0.631427 |
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Target: 5'- uGCCaGCCGagaagaaCGGcagcUCGCCGAaGCCGCCc -3' miRNA: 3'- -CGG-CGGC-------GCCu---AGCGGCUgUGGUGGc -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 27319 | 0.66 | 0.631427 |
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Target: 5'- uGCCGUCGCGGGUguauccgCGCUGAaGCUugUc -3' miRNA: 3'- -CGGCGGCGCCUA-------GCGGCUgUGGugGc -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 2656 | 0.66 | 0.63242 |
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Target: 5'- aCCGCCgGCGGcAUCGuaGAcCACCAgauCCGc -3' miRNA: 3'- cGGCGG-CGCC-UAGCggCU-GUGGU---GGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 43881 | 0.66 | 0.639368 |
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Target: 5'- aGCuCGCgCGCGaGAUcCGCCccgaagccgggaccGGCACCGuCCGg -3' miRNA: 3'- -CG-GCG-GCGC-CUA-GCGG--------------CUGUGGU-GGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 27517 | 0.66 | 0.641353 |
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Target: 5'- aGuuGaCCGCGGAuucggucUCGCacACACCAUCGc -3' miRNA: 3'- -CggC-GGCGCCU-------AGCGgcUGUGGUGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 73533 | 0.66 | 0.642345 |
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Target: 5'- gGCCaGCCGagcuUUGCCGcACGCCugCGa -3' miRNA: 3'- -CGG-CGGCgccuAGCGGC-UGUGGugGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 48979 | 0.66 | 0.642345 |
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Target: 5'- gGCCGCCcagcucGUGGAgaugcucggCGUCGACGuggaCACCGu -3' miRNA: 3'- -CGGCGG------CGCCUa--------GCGGCUGUg---GUGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 102454 | 0.66 | 0.642345 |
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Target: 5'- cGCauggGCCGCGaacGAUgagGCCGACGCUAUCGc -3' miRNA: 3'- -CGg---CGGCGC---CUAg--CGGCUGUGGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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