Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20454 | 3' | -61.6 | NC_004688.1 | + | 75580 | 0.73 | 0.245395 |
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Target: 5'- gGCCGCCGCGGAcuccUCcgGCUGACccgaGCCAUCc -3' miRNA: 3'- -CGGCGGCGCCU----AG--CGGCUG----UGGUGGc -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 33869 | 0.73 | 0.244822 |
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Target: 5'- cGCCGUUaccggcaccaccaGCGGGcgCGCCGugGCCGCCc -3' miRNA: 3'- -CGGCGG-------------CGCCUa-GCGGCugUGGUGGc -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 60812 | 0.75 | 0.207925 |
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Target: 5'- gGCCGCgGCGG-UCGCCaaugguccaGGCcCCGCCGg -3' miRNA: 3'- -CGGCGgCGCCuAGCGG---------CUGuGGUGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 4206 | 0.75 | 0.193437 |
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Target: 5'- -gCGCCGCGGG-CGCCGAgGCCcUCGg -3' miRNA: 3'- cgGCGGCGCCUaGCGGCUgUGGuGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 26400 | 0.75 | 0.184276 |
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Target: 5'- uGCCGCCGCcGAggcCGCCGGaGCUGCCGg -3' miRNA: 3'- -CGGCGGCGcCUa--GCGGCUgUGGUGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 22880 | 0.76 | 0.1755 |
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Target: 5'- cGCCGCCGCGG--CGCCuACcccuACCGCCGc -3' miRNA: 3'- -CGGCGGCGCCuaGCGGcUG----UGGUGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 41372 | 0.77 | 0.137 |
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Target: 5'- cGCCGCCGUaaacguaaGGAUUGCCGAC-CCGCg- -3' miRNA: 3'- -CGGCGGCG--------CCUAGCGGCUGuGGUGgc -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 30914 | 0.79 | 0.101101 |
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Target: 5'- cCCGCC-CGGAUCGaCCGGgguCACCACCGg -3' miRNA: 3'- cGGCGGcGCCUAGC-GGCU---GUGGUGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 32577 | 1.1 | 0.000686 |
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Target: 5'- cGCCGCCGCGGAUCGCCGACACCACCGu -3' miRNA: 3'- -CGGCGGCGCCUAGCGGCUGUGGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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