Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20454 | 3' | -61.6 | NC_004688.1 | + | 96540 | 0.67 | 0.582929 |
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Target: 5'- aCCGCCccacuucagucgGCGGGggccaGCCGACGCaauCCGa -3' miRNA: 3'- cGGCGG------------CGCCUag---CGGCUGUGgu-GGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 96610 | 0.7 | 0.402687 |
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Target: 5'- cCCGCC-CGGAcucgaaccgagacgCGCCGAaauUACCGCCGg -3' miRNA: 3'- cGGCGGcGCCUa-------------GCGGCU---GUGGUGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 98341 | 0.66 | 0.622494 |
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Target: 5'- aGCUcCCGCcaGUCGUCGACGCC-CCa -3' miRNA: 3'- -CGGcGGCGccUAGCGGCUGUGGuGGc -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 99864 | 0.7 | 0.40769 |
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Target: 5'- uGCCGCaucuGCuGGUgGCUGGCGCgACCGg -3' miRNA: 3'- -CGGCGg---CGcCUAgCGGCUGUGgUGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 100848 | 0.67 | 0.553581 |
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Target: 5'- uGCCGcCCGaCGGcaAUCGgaCGGCACCcgacACCGg -3' miRNA: 3'- -CGGC-GGC-GCC--UAGCg-GCUGUGG----UGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 102353 | 0.67 | 0.53426 |
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Target: 5'- gGCCGgucuaCUGCGaGggCGaCGACACCGCCa -3' miRNA: 3'- -CGGC-----GGCGC-CuaGCgGCUGUGGUGGc -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 102454 | 0.66 | 0.642345 |
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Target: 5'- cGCauggGCCGCGaacGAUgagGCCGACGCUAUCGc -3' miRNA: 3'- -CGg---CGGCGC---CUAg--CGGCUGUGGUGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 102936 | 0.72 | 0.314659 |
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Target: 5'- cGCCGCCGaGGAucggcuagagcuaUCGCgGGCGCgACUGg -3' miRNA: 3'- -CGGCGGCgCCU-------------AGCGgCUGUGgUGGC- -5' |
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| 20454 | 3' | -61.6 | NC_004688.1 | + | 110858 | 0.67 | 0.543893 |
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Target: 5'- aGCCGCCgGCGG-UCGaacauacuuUCGAC-CCGCCc -3' miRNA: 3'- -CGGCGG-CGCCuAGC---------GGCUGuGGUGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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