Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20455 | 5' | -49.1 | NC_004688.1 | + | 584 | 0.66 | 0.990885 |
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Target: 5'- cAGGcUCAugACCGGUGGGcCGuucUUGCg -3' miRNA: 3'- -UUC-AGUugUGGCUACUC-GCuu-AACGa -5' |
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| 20455 | 5' | -49.1 | NC_004688.1 | + | 93226 | 0.66 | 0.989512 |
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Target: 5'- -cGUgGGCACCGAccucGGCGAAggGCUc -3' miRNA: 3'- uuCAgUUGUGGCUac--UCGCUUaaCGA- -5' |
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| 20455 | 5' | -49.1 | NC_004688.1 | + | 64349 | 0.67 | 0.986288 |
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Target: 5'- uGAGUCAccagGCGCUGAgagGGGUGggUgccaUGCa -3' miRNA: 3'- -UUCAGU----UGUGGCUa--CUCGCuuA----ACGa -5' |
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| 20455 | 5' | -49.1 | NC_004688.1 | + | 3276 | 0.67 | 0.980092 |
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Target: 5'- uAGUCAACGCCGAgucGCGGg--GCa -3' miRNA: 3'- uUCAGUUGUGGCUacuCGCUuaaCGa -5' |
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| 20455 | 5' | -49.1 | NC_004688.1 | + | 104171 | 0.7 | 0.939562 |
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Target: 5'- -uGUCGACGCUGAUGGGUGGcucacGCg -3' miRNA: 3'- uuCAGUUGUGGCUACUCGCUuaa--CGa -5' |
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| 20455 | 5' | -49.1 | NC_004688.1 | + | 32945 | 1.05 | 0.013439 |
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Target: 5'- cAAGUCAACACCGAUGAGCGAAUUGCUc -3' miRNA: 3'- -UUCAGUUGUGGCUACUCGCUUAACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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