Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20456 | 3' | -53.5 | NC_004688.1 | + | 23518 | 0.66 | 0.933538 |
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Target: 5'- cGAGCCCggugGCGAga-UCGGGGAGgaaGGa -3' miRNA: 3'- uUUCGGGg---UGCUacaAGCCCCUUa--CC- -5' |
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| 20456 | 3' | -53.5 | NC_004688.1 | + | 40963 | 0.67 | 0.890712 |
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Target: 5'- --uGCCCCuucgggggaauAgGGUGggUCGGGGAcUGGa -3' miRNA: 3'- uuuCGGGG-----------UgCUACa-AGCCCCUuACC- -5' |
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| 20456 | 3' | -53.5 | NC_004688.1 | + | 52891 | 0.67 | 0.89001 |
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Target: 5'- gAAAGCCUuuCACGGgaugcaaUGUcUCGuGGGggUGGa -3' miRNA: 3'- -UUUCGGG--GUGCU-------ACA-AGC-CCCuuACC- -5' |
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| 20456 | 3' | -53.5 | NC_004688.1 | + | 40300 | 0.71 | 0.709767 |
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Target: 5'- aAAAGCCCCGCGcaccuugGUGguggCGGGGuuuucUGGg -3' miRNA: 3'- -UUUCGGGGUGC-------UACaa--GCCCCuu---ACC- -5' |
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| 20456 | 3' | -53.5 | NC_004688.1 | + | 27992 | 0.76 | 0.433199 |
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Target: 5'- -cGGCCCCAgauauCGAUGUaggcggcaUCGGGGAAcGGg -3' miRNA: 3'- uuUCGGGGU-----GCUACA--------AGCCCCUUaCC- -5' |
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| 20456 | 3' | -53.5 | NC_004688.1 | + | 33093 | 1.08 | 0.00401 |
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Target: 5'- aAAAGCCCCACGAUGUUCGGGGAAUGGu -3' miRNA: 3'- -UUUCGGGGUGCUACAAGCCCCUUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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