Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20463 | 3' | -55.7 | NC_004688.1 | + | 6499 | 0.66 | 0.879589 |
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Target: 5'- cGCCG-GCGAgGUCUggGCUGgGGUUGu -3' miRNA: 3'- -CGGCuUGCUgCGGAa-CGACaCCGACc -5' |
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| 20463 | 3' | -55.7 | NC_004688.1 | + | 51422 | 0.66 | 0.872268 |
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Target: 5'- uGuuGAGCG-CGCCaagGCUGc-GCUGGu -3' miRNA: 3'- -CggCUUGCuGCGGaa-CGACacCGACC- -5' |
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| 20463 | 3' | -55.7 | NC_004688.1 | + | 3490 | 0.68 | 0.787953 |
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Target: 5'- aGCUGGuCGGCGgCUUGCUGaUGGUg-- -3' miRNA: 3'- -CGGCUuGCUGCgGAACGAC-ACCGacc -5' |
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| 20463 | 3' | -55.7 | NC_004688.1 | + | 106304 | 0.68 | 0.778582 |
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Target: 5'- aUCGcGCGGCGCCUgggGUaUGUGGUgaUGGa -3' miRNA: 3'- cGGCuUGCUGCGGAa--CG-ACACCG--ACC- -5' |
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| 20463 | 3' | -55.7 | NC_004688.1 | + | 79976 | 0.69 | 0.729886 |
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Target: 5'- uGUCGAACGGauCGCCaUUGCgGUGGagggccaUGGg -3' miRNA: 3'- -CGGCUUGCU--GCGG-AACGaCACCg------ACC- -5' |
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| 20463 | 3' | -55.7 | NC_004688.1 | + | 6178 | 0.69 | 0.719846 |
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Target: 5'- cGCCGAAggUGugGCaguaggGCUGUGugcGCUGGu -3' miRNA: 3'- -CGGCUU--GCugCGgaa---CGACAC---CGACC- -5' |
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| 20463 | 3' | -55.7 | NC_004688.1 | + | 101051 | 0.69 | 0.709728 |
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Target: 5'- aCCGGAgGACGUCUcgggccUGCUGgcgaaGCUGGa -3' miRNA: 3'- cGGCUUgCUGCGGA------ACGACac---CGACC- -5' |
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| 20463 | 3' | -55.7 | NC_004688.1 | + | 99881 | 0.69 | 0.699542 |
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Target: 5'- cCUGAACcuggcuaaGAUGCCgcaucUGCUgGUGGCUGGc -3' miRNA: 3'- cGGCUUG--------CUGCGGa----ACGA-CACCGACC- -5' |
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| 20463 | 3' | -55.7 | NC_004688.1 | + | 30265 | 0.7 | 0.668672 |
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Target: 5'- aUCGAugguguACGGCGCCUUGUUGgugGGCa-- -3' miRNA: 3'- cGGCU------UGCUGCGGAACGACa--CCGacc -5' |
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| 20463 | 3' | -55.7 | NC_004688.1 | + | 36165 | 1.11 | 0.001607 |
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Target: 5'- gGCCGAACGACGCCUUGCUGUGGCUGGc -3' miRNA: 3'- -CGGCUUGCUGCGGAACGACACCGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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