miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20465 5' -54.3 NC_004688.1 + 18216 0.66 0.887133
Target:  5'- gGCCg-GUGauggGGCCcGUGGCGCCGGUGc -3'
miRNA:   3'- -CGGgaCAUg---CUGGuCACUGUGGUCAU- -5'
20465 5' -54.3 NC_004688.1 + 74958 0.68 0.81401
Target:  5'- uUCCUucuuuuguuggaGuUACGGCCGGUGAuuCGCCAGUAg -3'
miRNA:   3'- cGGGA------------C-AUGCUGGUCACU--GUGGUCAU- -5'
20465 5' -54.3 NC_004688.1 + 54295 0.72 0.600276
Target:  5'- uGCCCgcgGCGGCCGGggaACACCAGg- -3'
miRNA:   3'- -CGGGacaUGCUGGUCac-UGUGGUCau -5'
20465 5' -54.3 NC_004688.1 + 12511 0.74 0.496865
Target:  5'- aGCCCgagagGUGcCGACCGGUGACAUCuccgacGGUGu -3'
miRNA:   3'- -CGGGa----CAU-GCUGGUCACUGUGG------UCAU- -5'
20465 5' -54.3 NC_004688.1 + 36636 1.08 0.003002
Target:  5'- uGCCCUGUACGACCAGUGACACCAGUAc -3'
miRNA:   3'- -CGGGACAUGCUGGUCACUGUGGUCAU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.