Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20497 | 3' | -54.7 | NC_004688.1 | + | 63741 | 0.67 | 0.843318 |
Target: 5'- gGAGAGGUGAucgaucaCCGUGgagcgGGCCUCCGc -3' miRNA: 3'- -CUCUUCACUcc-----GGUACa----CUGGAGGCc -5' |
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20497 | 3' | -54.7 | NC_004688.1 | + | 65938 | 0.7 | 0.7214 |
Target: 5'- uGGggGUGAGGUUuUGUuuAUCUCCGGa -3' miRNA: 3'- cUCuuCACUCCGGuACAc-UGGAGGCC- -5' |
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20497 | 3' | -54.7 | NC_004688.1 | + | 64332 | 0.7 | 0.700884 |
Target: 5'- aGAGggGUGGGuGCCAUGcaGCUUCUGa -3' miRNA: 3'- -CUCuuCACUC-CGGUACacUGGAGGCc -5' |
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20497 | 3' | -54.7 | NC_004688.1 | + | 61024 | 1.1 | 0.002362 |
Target: 5'- cGAGAAGUGAGGCCAUGUGACCUCCGGc -3' miRNA: 3'- -CUCUUCACUCCGGUACACUGGAGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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