Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20499 | 3' | -52.7 | NC_004688.1 | + | 98804 | 0.66 | 0.937532 |
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Target: 5'- --uAGUCaGCGGGGGGcGGGCCagcgggcuggcuGACGCg -3' miRNA: 3'- uuuUUAG-CGCCCCUC-CUUGG------------CUGUGa -5' |
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| 20499 | 3' | -52.7 | NC_004688.1 | + | 48141 | 0.67 | 0.926872 |
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Target: 5'- cGAGAUCGCGGauuGGAugCGAgACg -3' miRNA: 3'- uUUUUAGCGCCccuCCUugGCUgUGa -5' |
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| 20499 | 3' | -52.7 | NC_004688.1 | + | 34488 | 0.67 | 0.926311 |
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Target: 5'- --cAAUCGCGGugaacaucuucguGGAGGAACCcAUGCg -3' miRNA: 3'- uuuUUAGCGCC-------------CCUCCUUGGcUGUGa -5' |
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| 20499 | 3' | -52.7 | NC_004688.1 | + | 75502 | 0.67 | 0.902388 |
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Target: 5'- -cAGGUCGCucGGGGuccAGGAGCCGAagACg -3' miRNA: 3'- uuUUUAGCG--CCCC---UCCUUGGCUg-UGa -5' |
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| 20499 | 3' | -52.7 | NC_004688.1 | + | 34901 | 0.69 | 0.849829 |
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Target: 5'- -cGAcgCGgGGGGAGGAacuggACCGAUcgGCg -3' miRNA: 3'- uuUUuaGCgCCCCUCCU-----UGGCUG--UGa -5' |
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| 20499 | 3' | -52.7 | NC_004688.1 | + | 96528 | 0.71 | 0.747003 |
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Target: 5'- --cAGUCgGCGGGGGccAGCCGACGCa -3' miRNA: 3'- uuuUUAG-CGCCCCUccUUGGCUGUGa -5' |
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| 20499 | 3' | -52.7 | NC_004688.1 | + | 7987 | 0.73 | 0.652257 |
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Target: 5'- -----cCGCGGGGAGGcgUCGAUugUg -3' miRNA: 3'- uuuuuaGCGCCCCUCCuuGGCUGugA- -5' |
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| 20499 | 3' | -52.7 | NC_004688.1 | + | 62785 | 1.05 | 0.007081 |
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Target: 5'- aAAAAAUCGCGGGGAGGAACCGACACUc -3' miRNA: 3'- -UUUUUAGCGCCCCUCCUUGGCUGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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