Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20499 | 5' | -60.3 | NC_004688.1 | + | 75613 | 0.66 | 0.670399 |
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Target: 5'- gGCAGcGCCGaa-CGAC-CCCUGCGACg -3' miRNA: 3'- -UGUU-CGGCcgaGCUGaGGGGCGCUGa -5' |
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| 20499 | 5' | -60.3 | NC_004688.1 | + | 47021 | 0.66 | 0.619676 |
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Target: 5'- uCGAGUCGGCUCGcaagaUCCgCGgGGCa -3' miRNA: 3'- uGUUCGGCCGAGCug---AGGgGCgCUGa -5' |
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| 20499 | 5' | -60.3 | NC_004688.1 | + | 7632 | 0.67 | 0.599397 |
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Target: 5'- --cGGCCGGUUCGcCUCgUCGCGGg- -3' miRNA: 3'- uguUCGGCCGAGCuGAGgGGCGCUga -5' |
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| 20499 | 5' | -60.3 | NC_004688.1 | + | 76205 | 0.67 | 0.579214 |
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Target: 5'- cGCAcGCCGGCaCGAUugccgucgucggUCCCCGCcACa -3' miRNA: 3'- -UGUuCGGCCGaGCUG------------AGGGGCGcUGa -5' |
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| 20499 | 5' | -60.3 | NC_004688.1 | + | 15372 | 0.68 | 0.539343 |
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Target: 5'- aGCAAGCUGGC-CGGCUaCUCGCcaaGACg -3' miRNA: 3'- -UGUUCGGCCGaGCUGAgGGGCG---CUGa -5' |
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| 20499 | 5' | -60.3 | NC_004688.1 | + | 91925 | 0.68 | 0.529514 |
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Target: 5'- cGCGAGCCGGUgaccagGAC-CCCCG-GGCa -3' miRNA: 3'- -UGUUCGGCCGag----CUGaGGGGCgCUGa -5' |
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| 20499 | 5' | -60.3 | NC_004688.1 | + | 14153 | 0.68 | 0.520725 |
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Target: 5'- cGCcguAGCgCGGCUCGGCgaugauguagaccgCCCGCGGCg -3' miRNA: 3'- -UGu--UCG-GCCGAGCUGag------------GGGCGCUGa -5' |
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| 20499 | 5' | -60.3 | NC_004688.1 | + | 26148 | 0.69 | 0.466535 |
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Target: 5'- gGCAAGCUGGC-CGagugcgccaccugccGCUCcaccgCCCGCGGCg -3' miRNA: 3'- -UGUUCGGCCGaGC---------------UGAG-----GGGCGCUGa -5' |
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| 20499 | 5' | -60.3 | NC_004688.1 | + | 27775 | 0.74 | 0.22649 |
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Target: 5'- -aGGGCCcGCcCGACUCCCCGuCGACa -3' miRNA: 3'- ugUUCGGcCGaGCUGAGGGGC-GCUGa -5' |
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| 20499 | 5' | -60.3 | NC_004688.1 | + | 62751 | 1.07 | 0.001083 |
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Target: 5'- aACAAGCCGGCUCGACUCCCCGCGACUg -3' miRNA: 3'- -UGUUCGGCCGAGCUGAGGGGCGCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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