Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20514 | 5' | -52.9 | NC_004688.1 | + | 74724 | 1.09 | 0.004166 |
Target: 5'- aGAACGUGGGGACACCUUUCCGGUUGAc -3' miRNA: 3'- -CUUGCACCCCUGUGGAAAGGCCAACU- -5' |
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20514 | 5' | -52.9 | NC_004688.1 | + | 89966 | 0.72 | 0.670705 |
Target: 5'- gGAAcCGUGGagaacGGCACCUUUCCGGUg-- -3' miRNA: 3'- -CUU-GCACCc----CUGUGGAAAGGCCAacu -5' |
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20514 | 5' | -52.9 | NC_004688.1 | + | 42000 | 0.67 | 0.923503 |
Target: 5'- uGGGCGgcGGGGuCGCCggUCCGGa--- -3' miRNA: 3'- -CUUGCa-CCCCuGUGGaaAGGCCaacu -5' |
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20514 | 5' | -52.9 | NC_004688.1 | + | 20010 | 0.66 | 0.939408 |
Target: 5'- gGGAUGaUGGGGGCGCCg--UCGGg--- -3' miRNA: 3'- -CUUGC-ACCCCUGUGGaaaGGCCaacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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