Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20516 | 3' | -55.4 | NC_004688.1 | + | 44582 | 0.66 | 0.882563 |
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Target: 5'- --aGGUAGUCGAcGgCGGGGCcgaucguGUACGu -3' miRNA: 3'- uagCUAUCAGCUaUgGCCCCG-------CGUGC- -5' |
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| 20516 | 3' | -55.4 | NC_004688.1 | + | 27885 | 0.67 | 0.844803 |
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Target: 5'- cGUCGccGUGGUCcugACCGuGGCGUACGg -3' miRNA: 3'- -UAGC--UAUCAGcuaUGGCcCCGCGUGC- -5' |
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| 20516 | 3' | -55.4 | NC_004688.1 | + | 108991 | 0.67 | 0.844803 |
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Target: 5'- uAUCGAUggacuccaugcGGUCGGUGCCaauuGGGCGgaACGa -3' miRNA: 3'- -UAGCUA-----------UCAGCUAUGGc---CCCGCg-UGC- -5' |
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| 20516 | 3' | -55.4 | NC_004688.1 | + | 29920 | 0.67 | 0.827952 |
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Target: 5'- -cCGAguagUGGUgCGAUAuuccacccguCCGGGGCGUACu -3' miRNA: 3'- uaGCU----AUCA-GCUAU----------GGCCCCGCGUGc -5' |
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| 20516 | 3' | -55.4 | NC_004688.1 | + | 53950 | 0.67 | 0.827952 |
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Target: 5'- --gGAUgcGGUCGGUGCgCaGGGCGUACu -3' miRNA: 3'- uagCUA--UCAGCUAUG-GcCCCGCGUGc -5' |
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| 20516 | 3' | -55.4 | NC_004688.1 | + | 19614 | 0.68 | 0.801298 |
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Target: 5'- -aCGAUG---GAUGCCGGGGCGaauaCGCGg -3' miRNA: 3'- uaGCUAUcagCUAUGGCCCCGC----GUGC- -5' |
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| 20516 | 3' | -55.4 | NC_004688.1 | + | 31480 | 0.68 | 0.773206 |
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Target: 5'- uGUCGcgGG-CGAgGCCGGGGUGgACc -3' miRNA: 3'- -UAGCuaUCaGCUaUGGCCCCGCgUGc -5' |
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| 20516 | 3' | -55.4 | NC_004688.1 | + | 17310 | 0.71 | 0.609767 |
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Target: 5'- cUCGAUGcg-GAUGCCGGGGauuGCGCGg -3' miRNA: 3'- uAGCUAUcagCUAUGGCCCCg--CGUGC- -5' |
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| 20516 | 3' | -55.4 | NC_004688.1 | + | 44967 | 0.71 | 0.588911 |
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Target: 5'- -cCGGUGGgcUCGuAUGCCGGGGCGUccACu -3' miRNA: 3'- uaGCUAUC--AGC-UAUGGCCCCGCG--UGc -5' |
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| 20516 | 3' | -55.4 | NC_004688.1 | + | 76226 | 1.06 | 0.003653 |
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Target: 5'- gAUCGAUAGUCGAUACCGGGGCGCACGc -3' miRNA: 3'- -UAGCUAUCAGCUAUGGCCCCGCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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