Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20517 | 3' | -53.2 | NC_004688.1 | + | 83125 | 0.7 | 0.803869 |
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Target: 5'- ----gCCGGcgCGCACCGCCuugCGAuGGa -3' miRNA: 3'- caauaGGCCuaGCGUGGUGG---GCUuCC- -5' |
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| 20517 | 3' | -53.2 | NC_004688.1 | + | 57217 | 0.7 | 0.794631 |
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Target: 5'- ----aCCGucGUCGCAUCACCCGGgcGGGg -3' miRNA: 3'- caauaGGCc-UAGCGUGGUGGGCU--UCC- -5' |
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| 20517 | 3' | -53.2 | NC_004688.1 | + | 89796 | 0.72 | 0.715943 |
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Target: 5'- -aUGUCgGGGcCGCGCCAgCCgGGAGGc -3' miRNA: 3'- caAUAGgCCUaGCGUGGU-GGgCUUCC- -5' |
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| 20517 | 3' | -53.2 | NC_004688.1 | + | 101905 | 0.74 | 0.579821 |
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Target: 5'- aGUUGUUCGaGGUCGCGCgcauaccaCGCCCGAcuGGGa -3' miRNA: 3'- -CAAUAGGC-CUAGCGUG--------GUGGGCU--UCC- -5' |
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| 20517 | 3' | -53.2 | NC_004688.1 | + | 25235 | 0.74 | 0.559024 |
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Target: 5'- --cGUuuGGAUCGCuaaggACCugCUGAAGGa -3' miRNA: 3'- caaUAggCCUAGCG-----UGGugGGCUUCC- -5' |
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| 20517 | 3' | -53.2 | NC_004688.1 | + | 76473 | 1.11 | 0.00333 |
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Target: 5'- cGUUAUCCGGAUCGCACCACCCGAAGGu -3' miRNA: 3'- -CAAUAGGCCUAGCGUGGUGGGCUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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