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miRNA: 3'- cCGGCGGCGA-GAUG--------GGCgUCCCUCgu -5'
miRNA: 3'- ccGGCGGCGAGa-------UGGGCG----UCCCuCGU- -5'
miRNA: 3'- cCGGCGGCgaGAugGGcGUCC-CUCGu -5'
miRNA: 3'- cCGGCgGCGAGaugGGCG---UCCCUCGu -5'
miRNA: 3'- -CCGGcGgCgAGAuGGGCGUCCCuCGU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 20537 | 5' | -62.8 | NC_004688.1 | + | 51958 | 0.66 | 0.499144 |
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Target: 5'- uGCCGCCGCUgCUGCgguuguagCCG-AGGGGGa- -3' miRNA: 3'- cCGGCGGCGA-GAUG--------GGCgUCCCUCgu -5' |
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| 20537 | 5' | -62.8 | NC_004688.1 | + | 19882 | 0.66 | 0.499144 |
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Target: 5'- -uCCGCCGUUCacguaggcGCCCGCcgucAGGGuGCGg -3' miRNA: 3'- ccGGCGGCGAGa-------UGGGCG----UCCCuCGU- -5' |
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| 20537 | 5' | -62.8 | NC_004688.1 | + | 531 | 0.66 | 0.508616 |
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Target: 5'- uGUCGCCGggCUugCCcCAGGuGAGCu -3' miRNA: 3'- cCGGCGGCgaGAugGGcGUCC-CUCGu -5' |
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| 20537 | 5' | -62.8 | NC_004688.1 | + | 100591 | 0.66 | 0.527781 |
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Target: 5'- cGCUGgCGCUCa--CCGCccuGGGGGGCu -3' miRNA: 3'- cCGGCgGCGAGaugGGCG---UCCCUCGu -5' |
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| 20537 | 5' | -62.8 | NC_004688.1 | + | 11293 | 0.66 | 0.537465 |
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Target: 5'- cGGCC-CgGgUCU-CCUGCAGGGcGCGc -3' miRNA: 3'- -CCGGcGgCgAGAuGGGCGUCCCuCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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