Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20537 | 5' | -62.8 | NC_004688.1 | + | 93422 | 0.69 | 0.368409 |
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Target: 5'- uGGCuCGCCGUUCUcgaacggcuGgUCGCAGGuaGAGCAc -3' miRNA: 3'- -CCG-GCGGCGAGA---------UgGGCGUCC--CUCGU- -5' |
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| 20537 | 5' | -62.8 | NC_004688.1 | + | 96824 | 0.69 | 0.337725 |
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Target: 5'- aGGUCGgCGUUCUGCCCGUGGuucggcgcccGGuGCAg -3' miRNA: 3'- -CCGGCgGCGAGAUGGGCGUC----------CCuCGU- -5' |
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| 20537 | 5' | -62.8 | NC_004688.1 | + | 98831 | 0.67 | 0.444106 |
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Target: 5'- cGGcCCGCgGCgacaaagaggaCUGCCUagucaGCGGGGGGCGg -3' miRNA: 3'- -CC-GGCGgCGa----------GAUGGG-----CGUCCCUCGU- -5' |
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| 20537 | 5' | -62.8 | NC_004688.1 | + | 100591 | 0.66 | 0.527781 |
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Target: 5'- cGCUGgCGCUCa--CCGCccuGGGGGGCu -3' miRNA: 3'- cCGGCgGCGAGaugGGCG---UCCCUCGu -5' |
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| 20537 | 5' | -62.8 | NC_004688.1 | + | 103664 | 0.71 | 0.269346 |
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Target: 5'- cGGCCgGCgGCUCUGCCgaGCucguGGuGAGCGu -3' miRNA: 3'- -CCGG-CGgCGAGAUGGg-CGu---CC-CUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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