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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
2054 | 3' | -55.7 | NC_001347.2 | + | 26583 | 0.74 | 0.581339 |
Target: 5'- cGUGGCUUGGgagcaccgUCuCAGCUCCGUguGg -3' miRNA: 3'- -UACCGGACCaua-----AG-GUCGAGGCAguC- -5' |
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2054 | 3' | -55.7 | NC_001347.2 | + | 180440 | 0.74 | 0.580335 |
Target: 5'- cGUGGCCUGGcccgaacgcugcuUcaUCCAGCUCCGUUc- -3' miRNA: 3'- -UACCGGACC-------------AuaAGGUCGAGGCAGuc -5' |
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2054 | 3' | -55.7 | NC_001347.2 | + | 39233 | 1.06 | 0.00643 |
Target: 5'- cAUGGCCUGGUAUUCCAGCUCCGUCAGc -3' miRNA: 3'- -UACCGGACCAUAAGGUCGAGGCAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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