Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20541 | 3' | -55.7 | NC_004688.1 | + | 85660 | 0.66 | 0.834777 |
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Target: 5'- ----uGGAGUUCgcCCcUGCCGCCGu -3' miRNA: 3'- cacuuCCUCGAGaaGGuACGGCGGCu -5' |
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| 20541 | 3' | -55.7 | NC_004688.1 | + | 44242 | 0.67 | 0.789777 |
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Target: 5'- -aGucGGGGC-CggCgAUGCCGCCGGu -3' miRNA: 3'- caCuuCCUCGaGaaGgUACGGCGGCU- -5' |
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| 20541 | 3' | -55.7 | NC_004688.1 | + | 8900 | 0.68 | 0.720712 |
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Target: 5'- -cGAAGGAcauUUCgUCCAUGUCGCCGc -3' miRNA: 3'- caCUUCCUc--GAGaAGGUACGGCGGCu -5' |
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| 20541 | 3' | -55.7 | NC_004688.1 | + | 23026 | 0.68 | 0.70941 |
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Target: 5'- gGUGAGGGcaaGGCUCUgagaggCCGUGacaacggcuuccaUCGCCGAu -3' miRNA: 3'- -CACUUCC---UCGAGAa-----GGUAC-------------GGCGGCU- -5' |
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| 20541 | 3' | -55.7 | NC_004688.1 | + | 33917 | 0.69 | 0.700099 |
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Target: 5'- ---cAGGAGCg---CCAuUGCCGCCGGa -3' miRNA: 3'- cacuUCCUCGagaaGGU-ACGGCGGCU- -5' |
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| 20541 | 3' | -55.7 | NC_004688.1 | + | 49390 | 0.72 | 0.512325 |
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Target: 5'- -aGAAuGGGGCg-UUCCAUGUCGCCGu -3' miRNA: 3'- caCUU-CCUCGagAAGGUACGGCGGCu -5' |
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| 20541 | 3' | -55.7 | NC_004688.1 | + | 91074 | 1.09 | 0.001988 |
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Target: 5'- gGUGAAGGAGCUCUUCCAUGCCGCCGAg -3' miRNA: 3'- -CACUUCCUCGAGAAGGUACGGCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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