Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20542 | 3' | -56.8 | NC_004688.1 | + | 8780 | 0.67 | 0.783473 |
Target: 5'- aGGUGC-CGGACUUGGuGCGGgccagcGGggUg -3' miRNA: 3'- -CUAUGaGCCUGAGCCcCGCCa-----CCuuG- -5' |
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20542 | 3' | -56.8 | NC_004688.1 | + | 103122 | 0.68 | 0.694828 |
Target: 5'- --aGCUCGccGGCUacUGGGGCGGUG-AGCa -3' miRNA: 3'- cuaUGAGC--CUGA--GCCCCGCCACcUUG- -5' |
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20542 | 3' | -56.8 | NC_004688.1 | + | 48338 | 0.69 | 0.653611 |
Target: 5'- --cAgUgGGGCUaCaGGGUGGUGGAGCa -3' miRNA: 3'- cuaUgAgCCUGA-GcCCCGCCACCUUG- -5' |
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20542 | 3' | -56.8 | NC_004688.1 | + | 32034 | 0.71 | 0.550459 |
Target: 5'- ---cCUCGGgaaACUCGGGGCGGgaaGAAUg -3' miRNA: 3'- cuauGAGCC---UGAGCCCCGCCac-CUUG- -5' |
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20542 | 3' | -56.8 | NC_004688.1 | + | 91453 | 1.09 | 0.001701 |
Target: 5'- aGAUACUCGGACUCGGGGCGGUGGAACu -3' miRNA: 3'- -CUAUGAGCCUGAGCCCCGCCACCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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