miRNA display CGI


Results 1 - 17 of 17 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20549 3' -51 NC_004688.1 + 79355 0.66 0.977793
Target:  5'- aCCU-CGUCCGCGGGUugGCGaacauGGCGAAa -3'
miRNA:   3'- -GGAuGUAGGCGUUUA--CGCc----CCGUUUa -5'
20549 3' -51 NC_004688.1 + 43730 0.66 0.97234
Target:  5'- gCCUGCGUagcCCGCGGGccaGGGGCGc-- -3'
miRNA:   3'- -GGAUGUA---GGCGUUUacgCCCCGUuua -5'
20549 3' -51 NC_004688.1 + 103483 0.66 0.971445
Target:  5'- gCCgagacCAUCCGCGAAUacGCGcgacagaucgagucGGGCGAGUa -3'
miRNA:   3'- -GGau---GUAGGCGUUUA--CGC--------------CCCGUUUA- -5'
20549 3' -51 NC_004688.1 + 43950 0.67 0.965981
Target:  5'- --cGCAUCgGCAugacgGcgGCGGGGUAGu- -3'
miRNA:   3'- ggaUGUAGgCGU-----UuaCGCCCCGUUua -5'
20549 3' -51 NC_004688.1 + 83177 0.67 0.965638
Target:  5'- gCC-ACAUCCGCGGGcgguuggggguguUGCGcGGGUGAu- -3'
miRNA:   3'- -GGaUGUAGGCGUUU-------------ACGC-CCCGUUua -5'
20549 3' -51 NC_004688.1 + 63884 0.67 0.958656
Target:  5'- gCCgACcgCgGUGAAUGCGGuGGCAAc- -3'
miRNA:   3'- -GGaUGuaGgCGUUUACGCC-CCGUUua -5'
20549 3' -51 NC_004688.1 + 51853 0.67 0.954616
Target:  5'- --gGCGUauggacCUGCGAAUGCGGucGGCAGAUg -3'
miRNA:   3'- ggaUGUA------GGCGUUUACGCC--CCGUUUA- -5'
20549 3' -51 NC_004688.1 + 99004 0.67 0.950316
Target:  5'- aCCcGCAccCCGgAAAUGCuGGGGCAc-- -3'
miRNA:   3'- -GGaUGUa-GGCgUUUACG-CCCCGUuua -5'
20549 3' -51 NC_004688.1 + 24290 0.68 0.945753
Target:  5'- gCCcGCGUCaGCAGGUGCcGGGCcGAUg -3'
miRNA:   3'- -GGaUGUAGgCGUUUACGcCCCGuUUA- -5'
20549 3' -51 NC_004688.1 + 58130 0.69 0.918895
Target:  5'- ---cCAUCCGCAccUGCGGGcGCGGc- -3'
miRNA:   3'- ggauGUAGGCGUuuACGCCC-CGUUua -5'
20549 3' -51 NC_004688.1 + 91154 0.69 0.905589
Target:  5'- gCCaACAUCgGCAAccacgaugugaucAUGCGGGGUg--- -3'
miRNA:   3'- -GGaUGUAGgCGUU-------------UACGCCCCGuuua -5'
20549 3' -51 NC_004688.1 + 52732 0.7 0.885285
Target:  5'- gCCg--AUCCGCGAAUGCugGGGGCc--- -3'
miRNA:   3'- -GGaugUAGGCGUUUACG--CCCCGuuua -5'
20549 3' -51 NC_004688.1 + 73503 0.7 0.845329
Target:  5'- gCCUugGUgcacgCCGCGcccuGcgGCGGGGCAGc- -3'
miRNA:   3'- -GGAugUA-----GGCGU----UuaCGCCCCGUUua -5'
20549 3' -51 NC_004688.1 + 17881 0.72 0.780425
Target:  5'- cUCUACGagCGCGAgacaGUGCGGcGGCGAGg -3'
miRNA:   3'- -GGAUGUagGCGUU----UACGCC-CCGUUUa -5'
20549 3' -51 NC_004688.1 + 51184 0.76 0.566981
Target:  5'- aCUGCAUCUGCGGGUGguCGGcGGCAGGc -3'
miRNA:   3'- gGAUGUAGGCGUUUAC--GCC-CCGUUUa -5'
20549 3' -51 NC_004688.1 + 39787 0.76 0.556242
Target:  5'- --gGCGUCUGCGAcUGCGGGGguGAg -3'
miRNA:   3'- ggaUGUAGGCGUUuACGCCCCguUUa -5'
20549 3' -51 NC_004688.1 + 95443 1.08 0.006585
Target:  5'- cCCUACAUCCGCAAAUGCGGGGCAAAUu -3'
miRNA:   3'- -GGAUGUAGGCGUUUACGCCCCGUUUA- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.