Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20557 | 5' | -63.9 | NC_004688.1 | + | 93479 | 0.66 | 0.456751 |
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Target: 5'- -gCGGcCGGGCGugaggUCGCACCaCGGGUg -3' miRNA: 3'- aaGCCcGCCCGC-----AGCGUGGgGCCUAa -5' |
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| 20557 | 5' | -63.9 | NC_004688.1 | + | 93247 | 0.66 | 0.456751 |
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Target: 5'- cUUUGGuGCGGGUGaUGCGCCCgUGGGc- -3' miRNA: 3'- -AAGCC-CGCCCGCaGCGUGGG-GCCUaa -5' |
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| 20557 | 5' | -63.9 | NC_004688.1 | + | 61758 | 0.66 | 0.411919 |
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Target: 5'- -gCGaGCGuGGUGcuucuucUCGCACCCCGGAa- -3' miRNA: 3'- aaGCcCGC-CCGC-------AGCGUGGGGCCUaa -5' |
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| 20557 | 5' | -63.9 | NC_004688.1 | + | 62884 | 0.67 | 0.395927 |
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Target: 5'- -gCGGGUGGGCGUCggagaGCGCCCaguagcaGGc-- -3' miRNA: 3'- aaGCCCGCCCGCAG-----CGUGGGg------CCuaa -5' |
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| 20557 | 5' | -63.9 | NC_004688.1 | + | 281 | 0.67 | 0.379531 |
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Target: 5'- uUUCGGG-GGGCGgcccCGCCCCGGu-- -3' miRNA: 3'- -AAGCCCgCCCGCagc-GUGGGGCCuaa -5' |
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| 20557 | 5' | -63.9 | NC_004688.1 | + | 98117 | 1.02 | 0.001084 |
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Target: 5'- uUUCGGGCGGGCGUCGCACCCCGGAUUc -3' miRNA: 3'- -AAGCCCGCCCGCAGCGUGGGGCCUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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